健脾益气止血方对脾气虚大鼠血清E_2、P及下丘脑、子宫ER、PR的影响  

Effects of Jianpiyiqizhixue Formula on the Estradiol and Progesterone in the Serum and on Estradiol and Progesterone Receptors in the Hypothalamus and Uterus in the Rat Model of Deficiency of Splenic Qi

作　　者：陈家旭[1] 陈易新[1] 季绍良[1] 瞿德竑[1] 徐洪雁[1] 唐已婷[1] 赵歆[1] 刘晓兰[1] 康纯洁[1] 刘燕[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《北京中医药大学学报》2002年第4期17-20,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家中医药管理局资助课题 (No .2 0 0 0 -J -B -2 0 ) ;高等学校学科博士专科点专项科研基金

摘　　要：探讨健脾益气止血方对脾气虚模型大鼠血清雌二醇 (E2 )、孕酮 (P)及下丘脑、子宫ER、PR的影响。以游泳加番泻叶水浸剂造脾气虚大鼠模型 ,用放射免疫分析方法检测血清E2 、P ,用免疫组织化学方法结合图像分析检测下丘脑及子宫ER、PR ,并观察健脾益气止血方对脾气虚大鼠模型的作用。健脾益气止血方可恢复脾气虚大鼠E2 、P的分泌水平 ,并使子宫、下丘脑ER水平升高 ,下丘脑PR进一步下降。健脾益气止血方可以改善脾气虚模型大鼠生殖系统功能。The effects of Jianpiyiqizhixue Formula (JF) on the levels of estradiol (E 2) and progesterone (P) in the serum and on the estradiol receptor (ER) and progesterone receptor (PR) in the hypothalamus and uterus were investigated in the rat model of deficiency of splenic qi. The model was established by inducement of swimming and gastric perfusion of senna water extract. The levels E 2 and P were detected by radioimmunoassay and the levels of ER and PR were measured by the immunohistochemical method and image analysis, in order to observe the effects of JF on the rat model of deficiency of splenic qi. The results showed that JF could restore the normal levels of E 2 and P, increase the level of ER in the hypothalamus and uterus, and decrease the level of PR in the hypothalamus in the rats of deficiency of splenic qi, which suggests that JF can improve the reproductive function of the rats of deficiency of splenic qi.

关 键 词：脾气虚 健脾益气止血方 雌激素受体 孕激素受体 大鼠 生殖系统功能 放射免疫分析法 

分 类 号：R289.5[医药卫生—方剂学]