同种异体移植大鼠耳廓组织中RANTES表达的实验研究  

Research of expression of RANTES in rat external ear allograft

作　　者：孙志成[1] 李世荣[1] 曹川[1] 郑红[2] 吴军[3] 

机构地区：[1]第三军医大学西南医院整形中心,重庆400038 [2]第三军医大学西南医院中心实验室,重庆400038 [3]第三军医大学西南医院烧伤研究所,重庆400038

出　　处：《重庆医学》2002年第8期663-665,共3页Chongqing medicine

基　　金：国家自然科学基金重大项目 (39993430 2 ) ;国家自然科学基金面上项目 (39970 756)

摘　　要：目的　探讨趋化因子RANTES在同种异体移植的大鼠耳廓组织中的表达与相关免疫细胞的浸润。方法　使用Lewis大鼠 8只作为供体 ,BN大鼠 8只作为受体 ,进行异体耳廓移植 (LewisBN) ;使用BN大鼠 16只进行自体移植 (BNBN)。观察移植耳廓的生存时间及病理改变 ,并采用免疫组织化学技术检测RANTES的表达与T淋巴细胞、单核细胞的浸润情况。结果　同种异体移植耳廓平均生存 (7 3± 0 2 5 )d ,移植耳廓组织中RANTES的表达于术后 3d达到高峰 ,并与组织中T淋巴细胞的浸润有明显的关系 ,同时可见大量单核细胞的浸润。结论　同种异体大鼠耳廓移植后 1周左右发生排斥反应 。Objective To study the expression of RANTES and recruitment of the related inflammatory cells in a model of rat external ear allotransplantation.Methods Rats were divided into two groups. One was the allo transplantion group, i.e., from Lewis to BN rats.The another group was the auto grafting, i.e, from BN to BN rats. The mean survival time of rat external ear, the pathological changes, the expression of RANTES and the recruitment of inflammatory cells were examed.Results The mean survival time of rat external ear allograft was 7 3±0 25days. Expression of RANTES in rat external ear allografts reached peak after operation 3 days and was apparently related with recruitment of T lymphocytes. Monocytes would also take party in rejection.Conclusion The rejection of rat external ear allografts occurr after transplantation about 1 week, which is statistically related with expression of RANTES and recruitment of T lymphocytes and monocytes.

关 键 词：同种异体移植 大鼠 耳廓组织 RANTES 趋化因子 免疫组织化学 排斥反应 

分 类 号：R392.4[医药卫生—免疫学]