胶束增敏荧光光度法测定土霉素的研究  被引量：6

Study on the determination of oxytetracycline by micellar enhanced spectrofluorimetry</em> <p class='article-op'> <span id="bdshare" class="bdshare_t bds_tools get-codes-bdshare baidufx"><span><span class="bds_more" style="display: block; width: 62px; height: 29px; background: url(/images/share.gif) no-repeat!important; padding: 0;"></span></span></span> </p> <p class="article-source"> <a href ='/article/read.aspx?id=6607366' class='btnTitle btnIsView' target='_blank'><img alt='在线阅读' src='/images/btn-read.png'/></a><a class='btnTitle btnIsView' href="javascript:showdown('v9JeBxPrE8nSQplxthVgySTu+VFKRnS9fptnCxmraa0=')"><img alt="下载全文" src="/images/btn-down.png" /></a> <input id="hiddowninfo" type="hidden" value="v9JeBxPrE8nSQplxthVgySTu+VFKRnS9fptnCxmraa0="/> </p> <div class="collect" style="display: none"> <h5>我的收藏</h5> <div class="cnt"> <p> 名 称：<input type="text" name="collTitle" id="collSumTitle" /> </p> <p> 注 释：<input type="text" name="collRemark" id="collSumRemark" /> </p> <p style="text-align: center;"> <a href="javascript:void(0)" onclick="g_collectSumOk();"> <img width="65" height="17" src="/images_new/collect_ok.png" alt="完成" /></a> <a href="javascript:void(0)" onclick="g_collectSumCancel();"> <img width="65" height="17" src="/images_new/collect_no.png" alt="取消" /></a> </p> </div> </div> </div> <form name="wxTransfer" method="post"> <input type="hidden" name="lid" value="" /> <input type="hidden" name="sip" value="" /> <input type="hidden" name="date" value="" /> <input type="hidden" name="co" value="" /> <input type="hidden" name="Title_C" value="" /> <input type="hidden" name="Writers" value="" /> <input type="hidden" name="Name_C" value="" /> <input type="hidden" name="Num" value="" /> <input type="hidden" name="Years" value="" /> <input type="hidden" name="Volumn" value="" /> <input type="hidden" name="GCH" value="" /> <input type="hidden" name="Vol" value="" /> <input type="hidden" name="BookID" value="" /> <input type="hidden" name="SubjectNum" value="" /> <input type="hidden" name="SpecialNum" value="" /> <input type="hidden" name="mirrorName" value="" /> <input type="hidden" name="UserID" value="" /> <input type="hidden" name="UserName" value="" /> <input type="hidden" name="sourceID" value="" /> </form> <script type="text/javascript" src="/scripts/transfer.min.js"></script> <div class="article-detail"> <p class='author'><strong>作　　者：</strong><a href='/writer/article.aspx?id=100000005307782' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e9%a9%ac%e7%ba%a2%e7%87%95%5d' title='马红燕[1]'>马红燕[1]</a> <a href='/writer/article.aspx?id=100000004738715' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e5%bc%a0%e7%a4%be%e4%ba%89%5d' title='张社争[1]'>张社争[1]</a> <a href='/writer/article.aspx?id=100000007491237' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e6%9b%b9%e5%b2%81%e5%b9%b3%5d' title='曹岁平[1]'>曹岁平[1]</a> <a href='/writer/article.aspx?id=100000007554474' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e6%9d%8e%e7%83%bd%5d' title='李烽[1]'>李烽[1]</a> <em> </em></p><p class='organ'><strong>机构地区：</strong><a href='/organ/article.aspx?id=59055&subid=&showname=&searchtype=' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=S%3d%e5%bb%b6%e5%ae%89%e5%a4%a7%e5%ad%a6%e5%8c%96%e5%ad%a6%e4%b8%8e%e5%8c%96%e5%b7%a5%e7%b3%bb+%e5%8f%8d%e5%ba%94%e5%b7%a5%e7%a8%8b%e7%9c%81%e7%ba%a7%e9%87%8d%e7%82%b9%e5%ae%9e%e9%aa%8c%e5%ae%a4' title='[1]延安大学化学与化工系 反应工程省级重点实验室,延安716000'>[1]延安大学化学与化工系 反应工程省级重点实验室,延安716000</a> <em> </em></p><p class='from'><strong>出　　处：</strong><a href='/journal/summary.aspx?gch=92500X' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=J%3d%5b%e5%88%86%e6%9e%90%e8%af%95%e9%aa%8c%e5%ae%a4%5d' title='分析试验室'>《分析试验室》</a>2002年第4期43-45,共3页<em>Chinese Journal of Analysis Laboratory</em></p><p class='fund'><strong>基　　金：</strong>陕西省教委专项科研基金(99JK124)资助课题</p><p class='abstrack'><strong>摘　　要：</strong>提出了表面活性剂增敏的酸性降解荧光法测定土霉素的新方法。土霉素与浓H2SO4反应,生成强荧光降解物一脱水土霉素,在pH10.0的NH3 NH4Cl缓冲溶液中,CTMAB的存在对脱水土霉素的荧光有较强的增敏和增稳作用,土霉素含量在1.21×10-8mol/L～4.83×10-6mol/L范围内与荧光强度成正比,方法的检出限为6 66×10-9mol/L。应用本法测定片剂中的土霉素,平均回收率为99.6%(n=5)。<em><Abstrcat>A new fluorimetric method, involving acidic degradation and surfactant enhancement,was proposed for determination of oxytetracycline. Oxytetracycline reacted with concentrated sulfuric acid and produced strong fluorescent species. With the presence of cetyltrimethyl ammonium bromide, in pH 10.0 NH3NH4Cl buffer solutions, the linear range for the determination of oxytetracycline were 1.21×10-8 mol/L～4.83×10-6 mol/L with determin limit of 6.66×10-9 mol/L. This methoed was applied to the determination of oxytetracycline in tablets with satisfactory results.</em></p><p class='subject'><strong>关 键 词：</strong><a href='/subject/article.aspx?id=5921745' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e8%83%b6%e6%9d%9f%e5%a2%9e%e6%95%8f%5d' title='胶束增敏'>胶束增敏</a> <a href='/subject/article.aspx?id=1085628' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e8%8d%a7%e5%85%89%e5%85%89%e5%ba%a6%e6%b3%95%5d' title='荧光光度法'>荧光光度法</a> <a href='/zk/search.aspx?key=K%3d%5b%e6%b5%8b%e5%ae%9a%5d' title='测定'>测定</a> <a href='/subject/article.aspx?id=2719154' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e5%9c%9f%e9%9c%89%e7%b4%a0%5d' title='土霉素'>土霉素</a> <a href='/subject/article.aspx?id=8744177' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e8%a1%a8%e9%9d%a2%e6%b4%bb%e6%80%a7%e5%89%82%5d' title='表面活性剂'>表面活性剂</a> <a href='/zk/search.aspx?key=K%3d%5b%e9%85%b8%e6%80%a7%e9%99%8d%e8%a7%a3%5d' title='酸性降解'>酸性降解</a> <a href='/subject/article.aspx?id=5187019' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e6%8a%97%e8%8f%8c%e7%b4%a0%5d' title='抗菌素'>抗菌素</a> <a href='/subject/article.aspx?id=871894' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e8%8d%af%e7%89%a9%e7%89%87%e5%89%82%5d' title='药物片剂'>药物片剂</a> </p><p class='class'><strong>分 类 号：</strong><a href='/zk/search.aspx?key=C%3dR978.14' title='R978.14'>R978.14</a>[<a href = '/domain/article.aspx?id=515' >医药卫生—药品</a>] <a href='/zk/search.aspx?key=C%3dR917' title='R917'>R917</a>[<a href = '/domain/article.aspx?id=509' >医药卫生—药学</a>] </p> </div> <div id="content_wzxg" name="wzxg" class="article-graph"> <object width="695" height="250" classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" id="FlashID"> <param value="../flash/relation.swf" name="movie"> <param value="high" 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