毛细管区带电泳法测定复方芦丁片中芦丁和维生素C的含量  被引量：7

Determination of rutin and vitamin C in compound rutin tablets using capillary zone electrophoresis</em> <p class='article-op'> <span id="bdshare" class="bdshare_t bds_tools get-codes-bdshare baidufx"><span><span class="bds_more" style="display: block; width: 62px; height: 29px; background: url(/images/share.gif) no-repeat!important; padding: 0;"></span></span></span> </p> <p class="article-source"> <a href ='/article/read.aspx?id=6607384' class='btnTitle btnIsView' target='_blank'><img alt='在线阅读' src='/images/btn-read.png'/></a><a class='btnTitle btnIsView' href="javascript:showdown('v9JeBxPrE8m56BxtqGGSW3bqeix4vY69nfp1dYY6HG0=')"><img alt="下载全文" src="/images/btn-down.png" /></a> <input id="hiddowninfo" type="hidden" value="v9JeBxPrE8m56BxtqGGSW3bqeix4vY69nfp1dYY6HG0="/> </p> <div class="collect" style="display: none"> <h5>我的收藏</h5> <div class="cnt"> <p> 名 称：<input type="text" name="collTitle" id="collSumTitle" /> </p> <p> 注 释：<input type="text" name="collRemark" id="collSumRemark" /> </p> <p style="text-align: center;"> <a href="javascript:void(0)" onclick="g_collectSumOk();"> <img width="65" height="17" src="/images_new/collect_ok.png" alt="完成" /></a> <a href="javascript:void(0)" onclick="g_collectSumCancel();"> <img width="65" height="17" src="/images_new/collect_no.png" alt="取消" /></a> </p> </div> </div> </div> <form name="wxTransfer" method="post"> <input type="hidden" name="lid" value="" /> <input type="hidden" name="sip" value="" /> <input type="hidden" name="date" value="" /> <input type="hidden" name="co" value="" /> <input type="hidden" name="Title_C" value="" /> <input type="hidden" name="Writers" value="" /> <input type="hidden" name="Name_C" value="" /> <input type="hidden" name="Num" value="" /> <input type="hidden" name="Years" value="" /> <input type="hidden" name="Volumn" value="" /> <input type="hidden" name="GCH" value="" /> <input type="hidden" name="Vol" value="" /> <input type="hidden" name="BookID" value="" /> <input type="hidden" name="SubjectNum" value="" /> <input type="hidden" name="SpecialNum" value="" /> <input type="hidden" name="mirrorName" value="" /> <input type="hidden" name="UserID" value="" /> <input type="hidden" name="UserName" value="" /> <input type="hidden" name="sourceID" value="" /> </form> <script type="text/javascript" src="/scripts/transfer.min.js"></script> <div class="article-detail"> <p class='author'><strong>作　　者：</strong><a href='/writer/article.aspx?id=100000006271663' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e4%bd%95%e9%a3%8e%e4%ba%91%5d' title='何风云[1]'>何风云[1]</a> <a href='/writer/article.aspx?id=100000007612111' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e6%9f%b3%e4%bb%81%e6%b0%91%5d' title='柳仁民[1]'>柳仁民[1]</a> <a href='/writer/article.aspx?id=100000009942752' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e5%88%98%e9%9b%aa%5d' title='刘雪[1]'>刘雪[1]</a> <em> </em></p><p class='organ'><strong>机构地区：</strong><a href='/organ/article.aspx?id=32825&subid=&showname=&searchtype=' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=S%3d%e8%81%8a%e5%9f%8e%e5%b8%88%e8%8c%83%e5%ad%a6%e9%99%a2%e5%8c%96%e5%ad%a6%e7%b3%bb' title='[1]聊城师范学院化学系,聊城252059'>[1]聊城师范学院化学系,聊城252059</a> <em> </em></p><p class='from'><strong>出　　处：</strong><a href='/journal/summary.aspx?gch=92500X' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=J%3d%5b%e5%88%86%e6%9e%90%e8%af%95%e9%aa%8c%e5%ae%a4%5d' title='分析试验室'>《分析试验室》</a>2002年第4期90-92,共3页<em>Chinese Journal of Analysis Laboratory</em></p><p class='fund'><strong>基　　金：</strong>.NULL.</p><p class='abstrack'><strong>摘　　要：</strong>考察了缓冲溶液的pH、背景电解质浓度及分离电压对芦丁、维生素C及苯甲酸分离的影响,建立了以苯甲酸为内标,毛细管区带电泳法快速测定复方芦丁片中芦丁和维生素C含量的新方法。以3g/L硼砂～4g/L硼酸(pH8 18)为运行缓冲液,苯甲酸为内标,在分离电压为25kV,检测波长为254nm的电泳条件下,芦丁、维生素C和内标可在5min实现分离。芦丁的线性方程为:y=2 65x+0 095(r=0 9994),线性范围为0 025mg/mL～0 4mg/mL,维生素C的线性方程为:y=3 1343x+0 565(r=0 9991),线性范围0 125mg/mL～1 0mg/mL。方法可用于复方芦丁片的质量控制。<em><Abstrcat> A capillary zone electrophoresis method for the determination of rutin and vitamic C in compound rutin tablet was established. The influences of the buffer pH, the concentration of the electrolyte and the separation voltage on the separation of rutin, vitamin C and benzonic acid were investigated.4 g/L boric acid～3 g/L borax(pH 818) was used as running buffers and benzoic acid as internal standard. The separation voltage was set at 25 kV and the detection wavelength at 254 nm. Under the optimum condition, rutin, vitamin C and internal standard could be well separated within 5 min. The calibration curve was linear in the range of 0.025 mg/mL～0400 mg/mL and 00625 mg/mL～100 mg/mL for rutin(r=0.9994) and vitamin C(r=09991), respectively.</em></p><p class='subject'><strong>关 键 词：</strong><a href='/subject/article.aspx?id=6865053' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e5%a4%8d%e6%96%b9%e8%8a%a6%e4%b8%81%e7%89%87%5d' title='复方芦丁片'>复方芦丁片</a> <a href='/subject/article.aspx?id=4625614' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e6%af%9b%e7%bb%86%e7%ae%a1%e5%8c%ba%e5%b8%a6%e7%94%b5%e6%b3%b3%5d' title='毛细管区带电泳'>毛细管区带电泳</a> <a href='/subject/article.aspx?id=3882878' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e7%bb%b4%e7%94%9f%e7%b4%a0C%5d' title='维生素C'>维生素C</a> <a href='/subject/article.aspx?id=4503573' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e8%8a%a6%e4%b8%81%5d' title='芦丁'>芦丁</a> <a href='/subject/article.aspx?id=870781' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e8%8d%af%e7%89%a9%e5%90%ab%e9%87%8f%e6%b5%8b%e5%ae%9a%5d' title='药物含量测定'>药物含量测定</a> </p><p class='class'><strong>分 类 号：</strong><a href='/zk/search.aspx?key=C%3dR977.2' title='R977.2'>R977.2</a>[<a href = '/domain/article.aspx?id=515' >医药卫生—药品</a>] <a href='/zk/search.aspx?key=C%3dR927.2' title='R927.2'>R927.2</a>[<a href = '/domain/article.aspx?id=509' >医药卫生—药学</a>] </p> </div> <div id="content_wzxg" name="wzxg" class="article-graph"> <object width="695" height="250" classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" id="FlashID"> <param value="../flash/relation.swf" name="movie"> <param value="high" name="quality"> <param value="opaque" name="wmode"> <param value="6.0.65.0" name="swfversion"> <param value="dataUrl=/ajax/articlerelativegraph.ashx?id=6607384" name="flashVars"> <embed width="695" height="250" src="../flash/relation.swf" 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