人血清脂蛋白与胆固醇修饰的葡聚糖的相互作用  

The Interaction of Human Lipoprotein with Cholesterol Modified Dextran in Serum

作　　者：曹宁宁[1] 俞耀庭[2] 王满燕[2] 王连永[2] 陈长治[2] 

机构地区：[1]天津理工学院生物与化学工程系 [2]南开大学分子生物研究所教育部生物活性材料重点实验室,天津300071

出　　处：《高等学校化学学报》2002年第8期1524-1528,共5页Chemical Journal of Chinese Universities

基　　金：国家重点基础研究与发展基金 (批准号 :9964 70 7);天津市高等学校科技发展基金项目 (批准号 :2 0 0 10 40 4)资助

摘　　要：采用浊度法和高效液相色谱法研究了人血清低密度脂蛋白 (LDL)和极低密度脂蛋白 (VLDL)与胆固醇修饰的葡聚糖 (CHD)的相互作用 .浊度法研究结果表明 ,CHD与 VLDL混合溶液的浊度大于 CHD与LDL混合溶液的浊度 .CHD浓度和 CHD链上胆固醇含量都对溶液的浊度产生影响 .当 CHD浓度为0 .3 mg/m L,CHD链上胆固醇含量为 4个 /1 0 0个糖环时 ,混合溶液的浊度可达到最大值 ,表明 LDL或VLDL聚集的程度最大 .Ca2 + 对 CHD与 LDL或 VLDL的相互作用没有影响 .高效液相色谱的研究结果表明 ,CHD与 LDL的结合使得复合物分子量增加 ;CHD分子量增大 ,复合物分子量也增加 ;当葡聚糖上胆固醇含量为 4.0时 ,复合物分子量最大 .实验结果表明 ,CHD能引起 LDL或The interaction of human low density lipoprotein(LDL) and very low density lipoprotein(VLDL) with cholesterol modified dextran(CHD) in serum was studied by turbidity and high performance liquid chromatography(HPLC)method. The solution turbidity of VLDL reacting with CHD was higher than that of LDL with CHD and was influenced by the concentration of CHD, cholesterol content of CHD and molecular weight of CHD . The turbidity reached the maximum value when the concentration of CHD was 0.3 mg/mL and cholesterol content was 4 cholesterol group/100 dextran glucose units. Ca 2+ had no influence on the interaction of VLDL and LDL with CHD.The molecular weights of LDL CHD complex were monitored by HPLC which showed that CHD molecules could associate multi molecules of LDL correlating with the results of turbidity experiments. The HPLC experiments showed that when cholesterol substitution number was 4/100 glucose units, the interaction of LDL with CHD reached the optimal condition and the complexation molecular weight increased with increasing dextran molecular weight. The experimental results show that CHD can induce self aggregation of LDL or VLDL.

关 键 词：人血清脂蛋白 胆固醇 修饰 葡聚糖 相互作用 低密度脂蛋白 极低密度脂蛋白 动脉粥样硬化 血液净化 治疗 吸附剂 

分 类 号：R541.4[医药卫生—心血管疾病] R457[医药卫生—内科学]