自组装膜中原位光反应固定化脱氧核糖核酸及膜稳定性  

Covalently Bonded DNA Multilayers Induced by In situ Photopolymerization and Their Stability

作　　者：侯学良[1] 孙璐[1] 吴立新[1] 张刚[1] 张希[1] 沈家骢[1] 

机构地区：[1]吉林大学超分子结构与材料教育部重点实验室,长春130023

出　　处：《高等学校化学学报》2002年第8期1601-1604,共4页Chemical Journal of Chinese Universities

基　　金：国家重点基础研究发展计划 (批准号 :G2 0 0 0 7810 0 );国家自然科学基金 (批准号 :2 9992 5 90 -5 )资助

摘　　要：通过静电沉积的方法构筑了含有脱氧核糖核酸 (DNA)和重氮树脂 (DAR)的交替多层膜 .在紫外光照射下 ,这种静电沉积多层膜相邻层间 DNA上的磷酸基团与 DAR上的重氮基团发生反应 ,从而将 DNA共价连接到多层膜中 .利用紫外 -可见吸收光谱和掠角反射吸收傅里叶变换红外光谱 (GRAFTIR)研究了这种相邻层间的光化学变化 .刻蚀实验结果发现 ,与光照前相比 。Electrostatic self assembled films of deoxyribonucleic acid(DNA) and diazoresin(DAR) were fabricated on substrates. Under irradiation, photoreaction occurring between adjacent layers was confirmed by UV Vis and Fourier transform infrared(FTIR) spectra, which covalently attached DNA to DAR in the multilayer. The stability of the multilayer was greatly improved, which was confirmed by etching in NaCl solution and monitored by UV Vis and scanning force microscopy(SFM).

关 键 词：自组装膜 原位光反应 固定化 脱氧核糖核酸 稳定性 静电沉积 共价连接 DNA膜 

分 类 号：Q523[生物学—生物化学]