FISH技术评价昆明山海棠在小鼠骨髓细胞中的非整倍体诱发效应  

ANEUPLOIDY INDUCTION BY THE WATER EXTRACT OF TRIPTERYGIUM HYPOGLAUCUM(LE-VEL) HUTCH IN MOUSE BONE MARROW CELLS DETECTED BY FISH

作　　者：丁银润[1] 王晓燕[1] 汪旭[1] 

机构地区：[1]云南师范大学生命科学学院遗传室,云南昆明650092

出　　处：《癌变．畸变．突变》2002年第3期144-147,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金 (3 9860 0 3 5 );云南省科委国际合作计划 (98C0 13 )

摘　　要：目的 :研究以荧光原位杂交 (fluorescenceinsituhybridization ,FISH)技术评价了中草药昆明山海棠根部水抽提物[Tripterygiumhypoglaucum (Level)Hutch ,THH]在小鼠骨髓细胞中的特异染色体不分离效应。方法 :以受试物THH腹腔注射昆明种雄性小白鼠 ,2 4h后取骨髓细胞常规制片 ,以bio 16 dUTP标记的 8号染色体特异性着丝粒重复顺序探针进行FISH ,并以streptavidine Cy3与杂交信号结合。荧光显微镜下分析受试物处理动物后骨髓细胞 8号染色体分离情况。结果 :在3个受试剂量中 ,THH具有与阳性对照秋水仙素类似的作用趋势 ,其所诱发的 8号染色体不分离频率均显著高于溶剂对照。结论 :本研究证实了THH为小鼠骨髓细胞 8号染色体异常分离的诱发因素。Purpose: The water extract of a Chinese herb, Tripterygium hypoglaucum (Level) Hutch (THH) was assayed for its effects of aneuploidy induction in mouse bone marrow cells by means of fluorescence in situ hybridization (FISH). Methods: Kunming species mice were injected with THH into the abdominal cavity and killed at 24 h after treatment. The bone marrow cell slides were prepared by general methods. FISH was carried out with Bio 16 dUTP labbled chromosome 8 probe. The hybridization signals were detected by combining streptavidine Cy3. Results: In three dose groups THH induced chromosome 8 aneuploidy and the aneuploidy frequencies of chromosome 8 were significantly higher than the solvent control (P<0.001～0.05). Conclusion: The result indicated that THH is an aneugen of chromosome 8 in mouse somatic cells.

关 键 词：荧光原位杂交 昆明山海棠 骨髓细胞 非整倍体 特异染色体不分离效应 

分 类 号：R285.5[医药卫生—中药学]