中缝背核注射对氯苯丙氨酸观察睡眠的变化及5-羟色胺神经元形态学的改变  

Effects of microinjection of PCPA into dorsal raphe nuclei on sleep and serotonergic neurons

作　　者：章功良[1] 赵乐章[1] 高隽[1] 张景行[1] 刘巧琼[1] 范一菲[1] 

机构地区：[1]安徽医科大学生理教研室,合肥230032

出　　处：《安徽医科大学学报》2002年第3期175-177,共3页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教委自然科研基金资助课题 (编号 :2 0 0 0J10 96);安徽医科大学校青年基金 (编号 :2 0 0 10 0 5 )

摘　　要：目的　观察大鼠中缝背核 (DRN)微量注射对氯苯丙氨酸 (PCPA)后睡眠的变化及 5 羟色胺 (5 HT)能神经元的形态学改变。方法　采用核团微量注射、多导睡眠描记和免疫组织化学技术。结果　DRN内微量注射PCPA(10 μg)引起睡眠时间增加 ,尤以深慢波睡眠增加明显 ;免疫组织化学显示DRN内 5 HT阳性神经元明显减少。结论　大鼠DRN微量注射PCPA后睡眠增加且 5 HT阳性神经元明显减少 ,提示 5 HT神经元参与睡眠调节。Objective To study the effects of microinjection of p chlorophenylalanine (PCPA,10 μg) into dorsal raphe nuclei(DRH) on sleep and serotonergic neurons. Methods Microinjection, polysomnography (PSG) and immunohistochemistrary were used in the experiment. Results Microinjection of PCPA into the DRN increase slow wave sleep (SWS) and paradoxical sleep (PS), and decreased wake (W) and the number of positive staining serotonergic neurons. Conclusion Microinjection of PCPA into rat DRN increases sleep and decreases the number of positive staining serotonergic neurons.

关 键 词：中缝背核 注射 对氯苯丙氨酸 睡眠 5-羟色胺 神经元形态学 

分 类 号：R338.63[医药卫生—人体生理学]