从早期胚胎分离和培养兔胚胎干细胞  

Isolation and culture of ES-like cell from rabbit preimplanted embryos

作　　者：周振明[1] 桑润滋[1] 韩建永[1] 孙国杰[1] 李俊杰[1] 

机构地区：[1]河北农业大学动物科技学院,河北保定071001

出　　处：《河北农业大学学报》2002年第3期70-73,共4页Journal of Hebei Agricultural University

基　　金：河北省科技攻关计划项目(0122041)

摘　　要：采用昆白系小鼠成纤维细胞(MEF)作为饲养层,低糖DMEM+10%NBS+10%FCS+10 ng/mL LIF+10 ng/mL SCF+0.1 mmol/L β-巯基乙醇+100 IU/mL青霉青+80 IU/mL链霉素作培养基,从培养96 h的胚胎中分离出1个兔类ES细胞集落,克隆传至4代,悬浮培养的兔类ES细胞团,可出现囊状胚。兔类ES细胞接种在衰老的饲养层上,出现滋养层细胞和上皮样细胞。One rabbit ES-like cell line was isolated from 96 h blastocysts by culture system composed of embryonic fibroblast feeder of Kun Ming strain white mouse (MEF),medium (low glucose DMEM, 10%NBS,10%FCS,0.1 mmol/L β-mercaptoethunol,100 IU/mLstreptomycin sulfate) supplied with 10 ng/mL SCF and 10 ng/mL LIF.One rabbit ES-like cell clones was passaged to 4 times.When ES-like cells were cultured on degenerated MEF,cells formed embryonic-like body.And also they differentiated epithelial and trophoblastic cells.

关 键 词：胚胎分离 兔 胚胎干细胞 内细胞团 胚胎培养 

分 类 号：Q78[生物学—分子生物学] S829.1[农业科学—畜牧学]