奶牛TNMD基因的克隆与原核表达  

Cloning and Prokaryotic Expression of Bovine TNMD Gene

作　　者：庞坤[1] 肖世文[1] 陈宏智[1] 易本驰[1] 刘纪成[1] 韩立强[2] 

机构地区：[1]信阳农林学院动物科学系,河南信阳464000 [2]河南农业大学牧医工程学院,郑州450002

出　　处：《湖北农业科学》2014年第7期1681-1683,共3页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(31201869);河南省教育厅科学技术研究重点项目(14A230013);河南省重大科技攻关项目(122101110100)

摘　　要：从奶牛组织中克隆Tenomodulin(TNMD)基因的cDNA序列,采用双酶切后连接表达载体pGEX-4T-1,转入大肠杆菌BL21中进行诱导表达。结果表明,TNMD基因的序列全长为957 bp,通过双酶切构建的表达载体pGEX-TNMD在BL21大肠杆菌中成功表达了分子量为59.68 kDa的融合蛋白。The Tenomodulin （TNMD﹚ cDNA of bovine was amplified by RT-CR. Then the gene fragment was digested with enzyme and cloned into expression vector pGEX-4T-1. The recombinant vector was transformed into E. coli BL21. The results showed that the full-length of the TNMD gene sequence was 957 bp. The reconstruction plasmid pGEX-TNMD was constructed successfully and a 59.68 kDa fusion protein was expressed in E. coli BL21 induced by IPTG.

关 键 词：奶牛 Tenomodulin(TNMD) 克隆 原核表达 

分 类 号：S823[农业科学—畜牧学] S961.6[农业科学—畜牧兽医]