大花序桉(Eucalyptus cloeziana)组培继代苗内源生根抑制物质定性分析  

Qualitative Analysis of Endogeneous Rooting Inhibitors of Eucalyptuscloeziana Tissue culture and Subculture

作　　者：黄永利 黄寿先[2] 谭健晖[3] 

机构地区：[1]南宁市林业科学研究所,广西武鸣530107 [2]广西大学,广西南宁530005 [3]广西林业科学研究院,广西南宁530002

出　　处：《广西师范学院学报（自然科学版）》2014年第2期48-51,共4页Journal of Guangxi Teachers Education University(Natural Science Edition)

基　　金：广西自然基金项目(2011GXNSFA018095)

摘　　要：以组培难生根的大花序桉(Eucalyptus cloeziana)和易生根尾巨桉继代苗为材料,用生物和化学显色方法鉴定大花序桉的内源生根抑制物质。结果表明:与尾巨桉相比,大花序桉继代苗提取液对绿豆和红豆的发芽无显著影响;大花序桉提取原液对绿豆插条发根率、发根量和根长均有明显的促进作用,尾巨桉GL9原液则有明显的抑制作用,差异达显著水平,提取液稀释1倍后无显著差异;两者提取液含黄酮类物质,不含烯醇结构酚类和皂甙,初步判断大花序桉组培生根的抑制物质可能为黄酮类物质。Rooting inhibitors of Eucalyptus cloeziana were studied with E .cloeziana rooting hard and E .urophylla ＆#215; E .grandis clone （GL9） rooting easily from biological effect and chemical assay , etc .Results showed that ,compared with GL9 ,the biological effect of E .cloez iana subculture extract was stronger ,E .cloeziana subculture seedlings extract had no significant effect of Mung bean and red bean sprout ;while the acidity and the main acid substance were similar ,and the difference for chemical assay of phenolic compounds was significant .The content of phenolic compounds in E .cloeziana extract was evidently greater by determination .Both phenolic compound that had inhibitive effect and phenolic compound that had promoting effect existed in the GL9 extract ,while there was only inhibi-tive monohydric phenolic in E .cloeziana cuttings by GC MS analysis .This preliminary indicated that the inhibition effect of acid substance on E .cloeziana cuttings rooting was not significant ,while the monohydric phenolic of phenolic compounds probably had certain inhibition effect on E .cloez iana cut-tings rooting .

关 键 词：大花序桉 生根抑制物 生物鉴定 化学显色 

分 类 号：S722.37[农业科学—林木遗传育种]