2012年邯郸市乙型Yamagata系流感病毒HA1基因变异分析  

Analysis of the characteristics of the HA1 gene of the Yamagata lineage of the influenza B virus isolated in 2012

作　　者：李燕霞[1] 郭瑞玲[1] 黄亮[1] 闫永飞[1] 邓健[1] 郭娜娜[1] 张治芳[1] 马燕霞[1] 李晓伟[1] 

机构地区：[1]邯郸市疾病预防控制中心急性传染病防制科,河北邯郸056008

出　　处：《中国病原生物学杂志》2014年第6期527-530,共4页Journal of Pathogen Biology

摘　　要：目的研究邯郸市2012年流感监测中分离的Yamagata系乙型流感毒株HA1的抗原性和基因特性，阐明HA1基因的变异与流感流行的关系。方法用MDCK细胞分离培养流感病毒，提取病毒核酸，采用RT—PCR方法特异性扩增病毒HA1基因，进行核苷酸序列测定，用DNAStar中Megalign软件进行分析。结果2012年分离的Yamagata系乙型流感病毒与2008-2009年代表株B／Florida／4／2。06相比亲缘关系较远，核苷酸同源性为96．2％～96．8％，氨基酸同源性为96．5％～97．4％，HA1区9个位点氨基酸发生替换，其中6个参与抗原表位构成；与WHO推荐的2012～2013年疫苗株B／Wisconsin／01／2010相比亲缘关系近，核苷酸同源性为98．8％～99．4％，氨基酸同源性为97．4％～98．5％，有5个位点发生氨基酸替换，其中3个参与抗原表位的构成；与国家流感中心提供的标准抗血清的血凝抑制滴度≥1：640。结论邯郸市2012年分离的Yamagata系乙型流感病毒血凝素蛋白HA1与B／Florida／4／2006相比已形成新的变种，与B／Wisconsin／01／2010相比也发生了新的变异，但尚不能确定是否形成Yamagata系有代表性的新变种。Objectives To study the characteristics of the HA1 gene of the Yamagata lineage of the influenza B virus isolated in the City of Handan in 2012, and to elucidate the relationship between the influenza epidemic/outbreak and variation in the HA1 gene. Methods Viruses were isolated and propagated using MDCK cells. The HA1 gene was ampli fled using a reverse transcription polymerase chain reaction （RT PCR）. The purified PCR products were sequenced. The data were analyzed with the software DNAStar. Results The B/Florida/4/2006 vaccine strain had a nucleotide similari- ty of 96.2%--96.8% to the influenza virus isolates from the City of Handan in 2012 and an amino acid similarity of 96. 5%--97.4%. Nine amino acids were substituted, 6 of which were located in the antigenic epitope. The influenza virus i- solates from the City of Handan had a nucleotide similarity of 98.8%--99.4% to B/Wisconsin/1/2010, which was the vaccine strain from 2012--2013 recommended by the WHO, and an amino acid similarity of 97.4%--98.5%. Five amino acids were substituted, 3 of which were located in the antigenic epitope. Conclusion The Yamagata lineage of the influ enza B virus isolated in 2012 is a new variant compared to B/Florida/4/2006 and has new mutations in comparison to B/ Wisconsin/i/2010. However, ibis study was unable to determine whether or not the influenza virus isolates from the City of Handan should be classified as a new variant typical of the Yamagata lineage.

关 键 词：流感病毒B型 Yamagata系 基因 序列分析 

分 类 号：R373.13[医药卫生—病原生物学]