滇西地区25种药用植物抗疟活性研究  

Evaluation of the antimalarial activity of 25 herbal medicines from west Yunnan

作　　者：肖朝江[1] 徐伟[1] 刘子琦[1] 彭俊霖 张小东[1] 姜北[1] 

机构地区：[1]大理学院药物研究所药学与化学学院,云南大理671000

出　　处：《中国病原生物学杂志》2014年第6期542-545,共4页Journal of Pathogen Biology

基　　金：云南省"百名海外高层次人才引进计划"项目(2011);云南省中青年学术技术带头人后备人才项目(No.2008PY005)

摘　　要：目的研究25种采自滇西地区药用植物的抗疟活性,为植物来源的新型抗疟药的研发天然产物奠定基础。方法依次用75%乙醇和水对25种药用植物进行回流提取,提取物经冷冻干燥后,采用β-羟高铁血红素形成抑制试验进行筛选,以IC50值评价其活性。结果在供试的25种药用植物中,苦参、滇白珠、马尾黄连等12种植物粗提物具有不同程度的β-羟高铁血红素形成抑制活性,其中苦参地上部分水提物和滇白珠提取物活性较好,IC50值分别为(1344.2±46.9)μg/ml和大于1 388.9μg/ml。具有抗疟活性的植物种类涉及10个科、12个属。结论苦参、滇白珠、马尾黄连等12种植物具有一定的抗疟活性,表明抗疟活性物质可能存在于多种植物中。Objectives To study the antimalarial activity of 25 herbal medicines from west Yunnan and to lay the foun-dation for the development of novel antimalarials from plants. Methods Plant materials were extracted with 75% etha nol and water using the reflux method. After the extract solutions were combined and concentrated under reduced pressure, the resulting residue was completely dried using lyophilization. The dried samples were screened for antimalarial activity using an assay of inhibition of 13 hematin formation. Bioactivity results were expressed as ICs0 values. Results Twelve plants including Sophora flavescens, Gaultheria leucocarpa, Thalictrum finetii, Leonurus japonicas, Phlomis betonicoides , Laggera pterodonta , Alternanthera pungens , Impatiens balsamina , Juncus setchuensis , Stellaria vestita , Caryopteris forrestii, and Clerodendrum bungei had moderate or weak activity to inhibit β-hematin formation. The aqueous extract of the aerial parts of S. flavescens had greater bioaetivity than the other herbal medicines, with an IC50 of （1,344.2± 46.9） μg/ml . The ethanol and aqueous extracts of G. leucocarpa had moderate bioactivity, with an IC5 0over 1388.9 μg/ml for both extracts, and the extracts had a respective inhibition rate of 46.0± 2.5 and 45.4± 1.7 at a concentration of 1 388.9 μg/ml. Plants with antimalarial activity came from 10 families and 12 genera, indicating that antimalarial constituents may broadly exist in plants. Conclusion Twelve of 25 tested plants warrant further study of their potential antimalarial activity.

关 键 词：抗疟疾活性 β-羟高铁血红素形成抑制实验 药用植物 粗提物 滇西地区 

分 类 号：R978.61[医药卫生—药品]