琼玉膏干预D-半乳糖致衰老大鼠的血清代谢组学研究  

Serum Metabonomic Study of Qiongyu Ointment in Treating D-galactose-induced Aging Rats

作　　者：刘焕兰[1] 曲卫玲[1] 武夏林[1] 胡霞[1] 曹媛[1] 

机构地区：[1]广州中医药大学基础医学院,广东广州510006

出　　处：《广州中医药大学学报》2014年第4期561-565,676,677,共7页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:81173318);广东省自然科学基金项目(编号:10151040701000058)

摘　　要：【目的】观察琼玉膏干预D-半乳糖致衰老大鼠血清代谢物变化,研究其抗衰老作用及机制。【方法】将36只大鼠随机平均分为3组,即空白对照组、模型组、琼玉膏组(剂量14 g·kg-1·d-1),采用皮下注射D-半乳糖(剂量为125 mg/kg)法复制衰老模型;给药结束后,腹主动脉采血,离心取血清,进行1H核磁共振检测,对样品进行主成分分析(PCA)、偏最小二乘法—判别分析(PLS-DA)、正交偏最小二乘法—判别分析(OPLS-DA)等,观察琼玉膏的抗衰老效果,筛选差异代谢物,分析代谢途径,探讨抗衰老机制。【结果】(1)PCA分析:优化度、预测度分别为R2X=80.8%,Q2=0.690,各组血清样本间有显著性差异;(2)PLS-DA分析:琼玉膏组与模型组比较有明显差异,拟合度、预测度分别为R2Y=0.931,Q2=0.826。(3)差异代谢物筛选:相关系数│r│>0.576有统计学意义,与空白对照组比较,模型组血清样本中有1-甲基组氨酸、醋酸盐等33种代谢物发生变化;与模型组比较,琼玉膏组有醋酸盐、缬氨酸等39种代谢物发生变化。【结论】琼玉膏延缓衰老的作用与糖代谢、氨基酸代谢等多条代谢通路有密切联系。Objective To observe the changes of serum metabolites of D-galactose--induced aging rats after intervention with Qiongyu Ointment （QO）, and to investigate the anti-aging effect and mechanism of QO. Methods Thirtysix rats were randomly divided into 3 groups, namely blank control group, model group, QO （ 14g·kg^-1·d^-1） group. Aging model was induced by subcutaneous injection of D-galactose in the dosage of 125 mg/kg. After the last administration, abdominal aortic blood was sampled and centrifuged, and then the isolated serum was analyzed by 1H nuclear magnetic resonance （NMR） . The results of the serum detection were analyzed with the methods of principal component analysis （PCA）, partial least squares discriminant analysis （PLS-DA）, and orthogonal partial least squares discriminant analysis （OPLS-DA）. According to the data, we screened the differential metabolites and analyzed the metabolic pathways, thus to explore the anti-aging mechanism of QO. Results （1） The results of PCA showed that optimization degree （R2X） was 80.8%, prediction value （Q2） was 0.690, and there were significant differences among the three groups. （2） The results of PLS-DA showed that fitting degree （WY） was 0.931 and Q2 was 0.826 between the model group and the QO group. （3） With correlation coeffi- cient over 0.576 as being statistical difference, 33 kinds of metabolites such as 1-methylhistidine and acetate in the model group differed from those in the blank control group, and 39 kinds of metabolites such as acetate and valine in QO group differed from those in the model group. Conclusion The anti-aging mechanism of QO has close relationship with the metabolic pathways of glucose metabolism and amino acid metabolism.

关 键 词：琼玉膏 药理学 衰老 中药疗法 代谢组学 血液 核磁共振 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]