肠和煎液对大鼠放射性肠炎的干预作用及机制  

Therapeutic Effect and Mechanism of Changhe Decoction for Rat Radiation Enterocolitis

作　　者：韦燕梅[1] 苏旭春[1] 孔嘉欣[1] 梁傍顺[1] 戴诗蕾 罗泽飞 

机构地区：[1]广州医科大学附属肿瘤医院,广东广州510095

出　　处：《广州中医药大学学报》2014年第4期582-586,679,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省中医药管理局基金资助项目(编号:20112161)

摘　　要：【目的】探讨肠和煎液对放射性肠炎大鼠的疗效及可能的作用机制。【方法】将46只雌性SD大鼠随机分为4组,即肠和煎液组（剂量为19.81 g·kg-1·d-1）、谷参肠安组（剂量为0.19 g·kg-1·d-1）、模型对照组、正常对照组。采用6MV医用X射线照射复制急性放射性肠炎大鼠模型,从照射后第1天开始,各组连续灌胃7 d。每日观察大鼠一般状况和排便情况,采用光学显微镜及图像分析系统观察、测定其相关的形态学指标,并检测小肠组织匀浆一氧化氮（NO）及白细胞介素-6（IL-6）的含量。【结果】照射后给药组一般状况及排便情况较模型对照组好,4~7 d对比明显;照射后第8天,与模型对照组比较,肠和煎液组大鼠体质量显著增加（P〈0.01）;肠和煎液组大鼠小肠绒毛高度、隐窝深度、黏膜及全层壁厚度较模型对照组均显著升高（P〈0.05）;与模型对照组比较,肠和煎液组、谷参肠安组小肠组织NO及IL-6含量均显著降低（P〈0.05或P〈0.01）,而肠和煎液组IL-6含量显著低于谷参肠安组（P〈0.05）。【结论】肠和煎液对大鼠小肠黏膜具有一定保护作用,可降低小肠组织炎性介质NO及炎症因子IL-6含量,可能是肠和煎液治疗急性放射性肠炎的机制之一。Objective To observe the therapeutic effect of Changhe Decoction （CD） for rat radiation enterocolitis and to explore the possible mechanism. Methods Forty-six female Sprague-Dawley rats were randomly divided into four groups, namely normal control group, model group, CD （19.81 g·kg^-1·d^-1） group and GlutaminEntersoluble Capsule （GEC, 0.19g·kg^-1·d^-1） group. Acute radiation enteritis rat model was established by exposure to a single total dose of 10 Gy radiated by 6 MV higher-energy X- rays on the abdominal region. From the first day of radiation, the rats were intragastrically administered with corresponding agents for 7 consecutive days.The rat general health status and defecation were observed everyday. At the end of experiment, intestinal histology was examined under light microscope by image analysis, and the intestinal contents of nitric oxide （NO） and interleukin-6 （IL-6） were also detected. Results After irradiation, general health condition and defecation were improved in CD group and GEC group as compared with those in the model group, especially on day 4-7. On day 8 after radiation, body mass was markedly increased in CD group （P〈0.01 compared with that in the model group） . Compared with the model group, intestinal villus height, intestinal crypt depth, intestinal mucosa thickness and entire intestinal wall thickness were obviously increased in CD group （P〈0.05）, and intestinal NO and IL-6 contents were markedly decreased in CD group and GEC group （P〈0.05 or P〈0.01） . CD group had lower IL-6 content than GEC group （P〈0.05） . Conclusion CD has protective effect against intestinal mucosal damage in acute radiation enteritis rat model. Reducing the intestinal NO and IL-6 contents may be one of its mechanisms for the treatment of acute radiation enteritis.

关 键 词：放射性肠炎 中药疗法 肠和煎液 药理学 小肠 病理学 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]