SFRP5在T2DM患者脂肪、肌肉中的表达及其与胰岛素抵抗的关系  

Expression of secreted frizzled related protein 5 in human adipose and muscle tissues and their relation with insulin resistance

作　　者：曾令海[1] 胡文静[2] 杨刚毅[2] 李伶[3] 王亚旭[1] 

机构地区：[1]重庆医科大学附属第二医院胃肠外科,400010 [2]重庆医科大学附属第二医院内分泌科,400010 [3]重庆医科大学检验系临床生化教研室和教育部实验诊断重点实验室

出　　处：《中华内分泌外科杂志》2014年第3期185-188,共4页Chinese Journal of Endocrine Surgery

基　　金：国家自然科学基金项目（30771037,81270913,81070640,30871199和30971388）

摘　　要：目的 探讨正常人及初诊2型糖尿病（type 2 diabetes mellitus,T2DM）患者脂肪、肌肉组织中分泌型卷曲相关蛋白5（secreted frizzled-related protein 5,SFRP5）的差异性表达及其与胰岛素抵抗（insulin resistance,IR）的关系.方法 按照1999年WHO的关于糖尿病的诊断标准选取初诊T2DM患者12例,及年龄、性别相匹配的正常人12例.所有研究对象均来自外科腹部择期手术患者,在同样手术条件下获取腹部大网膜脂肪组织和肌肉组织.常规测量体重、身高、腰围、臀围、血压、胰岛素（Fins）、血糖、糖化血红蛋白（HbA1C）、血脂等,计算胰岛素抵抗指数（HOMA-IR）、胰岛素敏感指数（HOMA-β）,用QRT-PCR方法和Western印迹方法检测SFRP5在不同人群中的差异性表达及其与IR的关系.结果 ①T2DM组与正常对照组相比,T2DM组收缩压（SBP）,甘油三酯（TG）,空腹血糖（FPG）,糖负荷后2h血糖（2h PG）,Fins,稳态模型评价HOMA-IR,HbA1C均升高.②T2DM组脂肪组织中SFRP5 mRNA和蛋白表达高于正常对照组,差异有统计学意义（2.7倍,5.5倍,均P＜0.01）,但在肌肉组织中差异无统计学意义.③脂肪组织SFRP5 mRNA和蛋白质高于肌肉组织.结论 脂肪组织SFRP5 mRNA和蛋白质表达高于肌肉组织提示脂肪组织可能是SFRP5的主要来源.T2DM组脂肪组织中SFRP5 mRNA和蛋白表达高于正常对照组提示SFRP5可能与IR严重程度相关.Objective To investigate the change expression levels of secreted frizzled related protein 5 （SFRP5） mRNA and protein in human adipose,and muscle tissues and the relationship between SFRP5 and insulin resistance in different glucose tolerance subjects.Methods Twelve type 2 diabetes mellitus（T2DM） patients and 12 aged and sex-matched healthy control subjects were enrolled.T2DM patients were diagnosed by1999 WHO criterion.Under the same conditions,the omental adipose and muscle tissues from these patients undergoing elective abdominal surgery were obtained.Expressions of sfrp5 protein and mRNA in adipose and muscle tissues were determined by semi—quantitive RT-PCR and Western blot while body height,weight,waist circumference,hipcircumference,blood pressure,lipodemia,fasting plasma glucose,fasting insulin,glycosylated hemoglobin,fasting serum insulin（FINS）,HOMA-β,HOMA-insulin resistance index（HOMA-IR） etc were investigated.Results 1.SBP,TG,FPG,2 h PG,Fins,HOMA-IR,HbA1C were significantly higher in T2DM groups than in the control group.2.The expressions of SFRP5 mRNA and protein in omental adipose tissues were significantly higher inT2DM group than in the control group（P ＜ 0.01）.3.The level of SFRP5 mRNA and protein expressions were higher in omental adipose tissue than in the muscle tissues.Conclusions The fact that mRNA and protein expression level of SFRP5 was higher in adipose tissue than in muscle indicates adipose tissue may be the main source of SFRP5.The expressions of SFRP5 mRNA and protein were higher inT2DM group than in the control group indicates sfrp5 may play a role in insulin resistance.

关 键 词：2型糖尿病 分泌型卷曲相关蛋白5 胰岛素抵抗 

分 类 号：R587.1[医药卫生—内分泌]