蓝靛果忍冬分化培养基的优化筛选  

Screening and optimization of Lonicera edulis differentiation culture

作　　者：李富恒[1] 曲迪[1] 赵恒田[2] 朱会杰[1] 杨洪升[1] 徐清华[1] 李楠丁 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030 [2]中国科学院东北地理与农业生态研究所,哈尔滨150081

出　　处：《东北农业大学学报》2014年第7期73-78,共6页Journal of Northeast Agricultural University

基　　金：国家外国专家局2013年度引进国外技术;管理人才项目(20132300046)

摘　　要：以蓝靛果忍冬优良品系L1-8为试验材料,取其休眠枝条萌发的腋芽为外植体,将培养基(MS、White、WPM、A3)、激素IBA(0.1、0.2、0.3、0.4 mg·L-1)、6-BA(1.0、1.5、2.0、2.5 mg·L-1)和GA3(0.5、1.0、1.5、2.0 mg·L-1)等处理组合进行4因素、4水平的正交试验,以优化筛选最佳分化培养基,为完善蓝靛果忍冬组培快繁技术提供技术支撑。结果表明,筛选出最优分化培养基为MS+6-BA 2.0 mg·L-1+IBA 0.3 mg·L-1+GA3 1.5mg·L-1(分化率可达95.92%);基本培养基和不同激素对分化率产生影响,在4个因素中,基本培养基和6-BA是影响分化率的主要因素,IBA和GA3为次要因素;筛选出的不同培养基组合对增殖系数影响不同,最高为4.14,且芽苗生长状况良好;继代周期为30d时,增殖效果最好。Lonicera edulis strain, designated strain L1-8 that provided by Chinese Academy of Science, Northeast Institute of Geography and Agricultural Ecology Institute , was applied as material in this research. The dormant branches of axil ary buds were used as explants for germination. The medium （MS, White, WPM, A3）, hormone IBA （0.1, 0.2, 0.3, 0.4 mg·L^-1）, 6-BA （1.0, 1.5, 2.0, 2.5 mg·L^-1） and GA3 （0.5, 1.0, 1.5, 2.0 mg·L^-1） were applied for the four factors-four levels orthogonal experiment which in order to optimize the best differentiation medium and provide technical support for the perfection of tissue culture technology of Lonicera edulis micropropagation. The results showed that MS medium containing 2.0 mg·L^-1 6-BA , 0.3 mg·L^-1 IBA and 1.5 mg·L^-1 GA3（The differentiation rate was 95.92%）was screened as the optimum differentiation medium. The basic medium and the different hormone both could affect the differentiation rate. In the four factors, the basic medium and 6-BA were the main factors affected the differentiation rate, whereas IBA and GA3 were the secondary factors. Screened on different culture combinations had different effects on multiplication. The multiplication was as high as 4.14 with the best growth. Subculture cycle was 30 d and the effect on multiplication was the best.

关 键 词：蓝靛果忍冬 分化 培养基 正交试验 增殖系数 

分 类 号：S663.9[农业科学—果树学]