生姜在大鼠脏腑组织分布与其归经相关性研究  

Correlation Studies of Shengjiang on the Distribution of the Viscera Tissue Distribution and Its Channel Entry

作　　者：李乾胜 申玲玲[1] 马开[2] 崔瑛[1] 王君明[1] 冯志毅[1] 汪晶[1] 

机构地区：[1]河南中医学院,河南郑州450046 [2]河南省中医药研究院,河南郑州450004

出　　处：《中医学报》2014年第7期1004-1009,共6页Acta Chinese Medicine

基　　金：郑州市科技领军人才项目(编号:121PLJRC534);河南中医学院科技创新团队计划项目(编号:2011XCXTD01)

摘　　要：目的:观察生姜的有效成分6-姜酚、6-姜烯酚、8-姜酚在脾胃虚寒组和正常组大鼠体内组织分布,探讨生姜的归经。方法:通过建立大鼠脾胃虚寒模型,评价生姜温中作用;采集脾胃虚寒组和正常组血清及组织样品,采用HPLC色谱法对6-姜酚、6-姜烯酚、8-姜酚在大鼠体内血药浓度和其在各脏腑的含量进行测定。结果:生姜对脾胃虚寒模型具有显著改善作用;6-姜酚在组织中分布浓度从高到低依次是:胃、小肠、大肠、肺、脾、肝、肾、心,脾胃虚寒组较正常组血清中浓度为高;6-姜烯酚、8-姜酚在大鼠血清中能检测到,在正常组的组织中未见分布,在脾胃虚寒组中分布于胃、小肠、大肠、脾;6-姜酚、6-姜烯酚、8-姜酚在脾胃虚寒组的组织分布对胃、小肠、大肠具有明显的趋向性。结论:生姜主要归胃(小肠、大肠、脾)经。其归经的物质基础为以6-姜酚、6-姜烯酚、8-姜酚为代表的生姜酚类成分。Objective： Through the tissue distribution of active components of Shengjiang such as 6-gingerol,6-shogaol and 8-gingerol in deficiencycold of the spleen group,explore the channel entry of Shengjiang. Methods： Establishing the model rats of deficiencycold of the spleen to evaluate the warming spleen-stomach effect of Shengjiang. Collect serum and tissue samples and use HPLC chromatography to evaluate the concentration of 6-gingerol,6-shogaol and 8-gingerol. Results： Shengjiang has significant improvement for deficiencycold of the spleen model. 6-gingerol concentration distribution in the organization from high to low in turn is： stomach,small intestine,colon,lung,spleen,liver,kidney,heart. The concentration in deficiencycold of the spleen group is higher. 6-shogaol and 8-gingerol can be detected in serum of rats but no in organization. In the deficiencycold of the spleen group,mainly distribute in the stomach,small intestine,large intestine and spleen. The distribution of 6-gingerol,6-shogaol and 8-gingerol in deficiencycold of the spleen group have obvious tendency on stomach,small intestine and large intestine. Conclusion： Shengjiang is mainly to the stomach channel entry. The material basis were maily 6-gingerol,6-shogaol and 8-gingerol represented by ginger phenolic components.

关 键 词：生姜 6-姜酚 6-姜烯酚 8-姜酚 组织分布 归经 

分 类 号：R285.5[医药卫生—中药学]