云南翠雀花根中总黄酮提取方法的研究  

Study on the Extraction Method Total Flavonoids in Root of the Delphinium yunnanense

作　　者：张虹[1] 王景李 白红丽[2] 李春燕[1] 袁寒[1] 郭俊明[2] 

机构地区：[1]红河学院生命科学与技术学院、云南省高校农作物优质高效栽培与安全控制重点实验室,云南蒙自661100 [2]云南民族大学、民族药资源化学国家民委一教育部重点实验室,云南昆明650500

出　　处：《北方园艺》2014年第13期153-154,共2页Northern Horticulture

基　　金：国家民族事务委员会教育部共建民族药资源化学重点实验室开放基金资助项目(MZY1405)

摘　　要：以云南翠雀花根为试材,采用索氏提取法、冷浸法和水提法,分别以乙醇、甲醇和蒸馏水作为提取剂,对云南翠雀花根中的总黄酮进行提取工艺研究。结果表明:云南翠雀花根中总黄酮提取量最高的方法为:索氏提取法,其次为冷浸法,最低为水提法;提取剂效果依次为:乙醇>甲醇>蒸馏水,以乙醇浓度为70%时的提取量最高为2.71mg/g。Root of the Delphinium yunnanense were used as materials,the total flavonoids in root of the Delphinium yunnanense were extracted by Soxhlet extraction,water extraction,cold-maceration using alcohol,methyl alcohol and distilled water as solvent.The results showed that the extraction of total flavonoids was the highest by Soxhlet extraction,followed by cold-maceration,the extraction of total flavonoids was the lowest by water extraction.The extraotion effects order was alcohol〉methyl alcohol〉distilled water.The alcohol 70%as extractant,the extraction effect of total flavonoids was the highest 2.71mg/g.

关 键 词：云南翠雀花根 总黄酮 提取 研究 

分 类 号：Q946[生物学—植物学]