化痰祛湿活血方对非酒精性脂肪性肝炎大鼠肝脏病理的影响  

Effects of Huatan Qushi Huoxue Formula on Hepatic Pathological Changes in Nonalcoholic Steatohepatitis Model Rats

作　　者：赵文霞[1] 闫乐[1] 段荣章[2] 

机构地区：[1]河南中医学院第一附属医院,河南省郑州市450000 [2]河南中医学院

出　　处：《中医杂志》2014年第14期1223-1226,共4页Journal of Traditional Chinese Medicine

基　　金：郑州市创新型科技领军人才项目(10LJRC171);河南中医学院科技创新团队项目(2010XCXTD06)

摘　　要：目的探讨化痰祛湿活血方对非酒精性脂肪性肝炎(NASH)大鼠肝组织病理学的影响。方法72只SD大鼠随机分为空白组、模型组、对照组和中药高、中、低剂量组,每组12只。空白组给予普通饲料,剩余各组均给予高脂饲料联合盐酸四环素腹腔注射建立NASH大鼠模型。从造模第2周起,对照组给予多烯磷脂酰胆碱胶囊混悬液28.5mg/(100g·d)灌胃;中药高、中、低剂量组分别给予化痰祛湿活血方混悬液5.0、2.52、1.26g/(100g·d)灌胃;正常组、模型组给予等剂量生理盐水灌胃。5周后测定各组大鼠血清丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、甘油三酯(TG)、总胆固醇(TC)水平,肝组织切片行HE及油红O染色,观察肝组织病理及脂肪沉积面积变化。结果与模型组比较,对照组和中药高、中、低剂量组治疗后血清ALT、AST、TG、TC水平均显著下降(P<0.01),且中药高剂量组降低血清ALT、AST、TG、TC水平更显著(P<0.05或P<0.01)。给药各组与模型组比较,肝细胞脂肪沉积的面积显著缩小或减少(P<0.01),并且以中药高剂量组效果最优(P<0.01)。结论化痰祛湿活血方能有效减轻肝脏损伤,改善肝细胞脂肪沉积,且以高剂量效果最优。Objective To study the effects of Huatan Qushi Huoxue Formula （formula to resolve phlegm, eliminate dampness and activate blood circulation） on pathological changes of liver tissue in rats with nonalcoholic steatohepatitis （NASH）. Methods Totally 72 male SD rats were randomized into the blank group, model group, control group and Huatan Qushi Huoxue Formula high-dose, middle-dose and low-dose groups, with 12 in each. The blank group was given normal diet. Other 5 groups were given fat diet and intraperitoneal injection of tetracycline to establish NASH models. From the 2nd week of modeling, the control group was gavaged with 28.5mg/（ 100g · d） polyene phosphatidylcholine capsule （Essentiale） suspension, Huatan Qushi Huoxue Formula high-dose, middle-dose and low-dose groups were gavaged with 5.0g/（100g · d）, 2. 52g/（100g · d） and 1.26g/（100g · d） ofHuatan Qushi Huoxue Formula suspension respectively, and the blank group and model group were gavaged with the equal volume of normal saline. The levels of alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, triglyceride （TG） and total cholesterol （TC） were detected 5 weeks later. The liver tissue sections Were stained by HE staining and oil red O staining. The pathological changes of liver tissue and changes of fat deposition area were observed. Results Comparing with the model group, the levels of ALT, AST, TG and TC were significantly decreased in the control group and Huatan Qushi Huoxue Formula high-dose, middle-dose and low-dose groups （P 〈 0.01 ）, especially in the Huatan Qushi Huoxue Formula high-dose group （P 〈 0.01 ）. Comparing with the model group, the area of fat deposition and inflammatory cell infiltration in the liver cells was significantly reduced in all administration groups （P 〈 0.01 ）, especially in the Huatan Qushi Huoxue Formula high-dose group. Conclusion Huatan Qushi Huoxue Formula can effectively reduce liver injury and relieve fat deposition in the liver cells, espe

关 键 词：非酒精性脂肪性肝炎 化痰祛湿活血方 肝脏病理 

分 类 号：R285.5[医药卫生—中药学]