氯离子通道阻断剂对大鼠高肺血流性肺动脉高压的影响  

Effect of chloride channel blocker on pulmonary hypertension induced by high pulmonary blood flow in rats

作　　者：马建法[1] 庞玉生[1] 陈传斯[2] 王凯[1] 潘宣任 唐巧云[1] 

机构地区：[1]广西医科大学第一附属医院儿科,南宁530021 [2]广西壮族自治区妇幼保健院

出　　处：《中华实用儿科临床杂志》2014年第13期993-996,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金（81160040）;广西自然科学基金（2013GXNSFAA019177）

摘　　要：目的 研究氯离子通道阻断剂尼氟灭酸（NFA）对大鼠高肺血流性肺动脉高压的作用.方法 雌雄不限的SD大鼠50只,随机数字表法分为5组：正常组、假手术组、模型组、给药1组、给药2组,每组10只.腹主动脉下腔静脉分流术后所有大鼠在相同条件下饲养11周,分别测定肺动脉平均压（mPAP）,右心室肥厚指数（RVHI）,肺动脉管壁面积与管总面积比（W/V）、管壁厚与管外径比（T/D）.结果 1.模型组的mPAP[（25.79±4.03） mmHg（1 mmHg=0.133 kPa）]均高于正常组[（16.48±1.70） mmHg]、假手术组[（17.03±2.01） mmHg]、给药1组[（21.78 ±2.77） mmHg]及给药2组[（20.31±2.15）mmHg] （F=18.983,P＜0.01）.给药1组的mPAP较给药2组略高,但差异无统计学意义（P＞0.05）;给药组的mPAP均高于正常组及假手术组,差异有统计学意义（P均＜0.01）.2.模型组的W/V及T/D均高于正常组、假手术组、给药1组及给药2组,差异有统计学意义（F =26.135、15.527,P均＜0.001）.给药1组与给药2组的W/V及T/D相比差异均无统计学意义（P均＞0.05）,但较正常组及假手术组高,差异有统计学意义（P均＜0.01）.结论 氯离子通道阻断剂NFA能部分降低高肺血流性肺动脉高压大鼠的mPAP,抑制血管平滑肌细胞增殖,提示NFA对高肺血流性肺动脉高压有一定的治疗作用.Objective To study the effect of chloride channel blocker（niflumic acid,NFA） on pulmonary hypertension induced by high pulmonary blood flow in rats.Methods Fifty male or female Sprague-Dawley rats were randomly divided into 5 groups：normal group,sham group,model group,drug 1 group,and drug 2 group,with 10 rats in each group.After subjected to an abdominal aorta-inferior vena cava shunt,all the rats were reared under the same condition for 11 weeks.Then,mean pulmonary artery pressure（mPAP） and right ventricular hypertrophy index（RVHI） of each rat were measured.In addition,arterial wall area/vessel area （W/V） and arterial wall thickness/vessel external diameter（T/D） of each rat were also measured.Results 1.The mPAP of model group [（25.79 ± 4.03） mmHg,1 mmHg =0.133 kPa] was significantly higher than those of normal group [（16.48 ± 1.70） mmHg],sham group [（17.03 ± 2.01） mmHg],drug 1 group [（21.78 ± 2.77） mmHg] and drug 2 group [（20.31 ± 2.15） mmHg] （F =18.983,P ＜0.01）.Although the mPAP of drug 1 group was a little higher than drug 2 group,there was no significant difference （P ＞ 0.05）.Compared with normal group and sham group,the mPAP of drug 1 group and drug 2 group increased（P ＜0.01,respectively）.2.The W/V and T/D of model group were significantly higher than those of normal group,sham group,drug 1 group and drug 2 group （F =26.135,15.527,all P ＜ 0.001）.The W/V and T/D of two drug groups showed no significant difference,but they were higher than those of normal group and sham group （P ＜ 0.01,respectively）.Conclusions Chloride channel blocker NFA partly decrease mPAP of pulmonary hypertension indnced by high pulmonary blood flow in rats,and inhibit proliferation of vascular smooth muscle cells.These results suggest that NFA had part of therapeutic effect to pulmonary hypertension induced by high pulmonary blood flow.

关 键 词：尼氟灭酸 高肺血流性肺动脉高压 肺动脉平滑肌细胞 氯离子通道 

分 类 号：R544.1[医药卫生—心血管疾病]