高盐饮食诱导Dah1大鼠高血压模型肾脏内源性硫化氢体系的变化  

Change of the renal hydrogen sulfide system in salt-sensitive hypertensive rats

作　　者：黄盼[1] 王素霞[2] 任雅丽[2] 唐朝枢[3] 杜军保[1] 金红芳[1] 

机构地区：[1]北京大学第一医院儿科,100034 [2]北京大学第一医院电镜室,100034 [3]北京大学医学部生理与病理生理学系

出　　处：《中华实用儿科临床杂志》2014年第13期997-1000,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：国家重点基础研究发展计划（973）（2012CB517806）

摘　　要：目的 在高盐饮食诱导盐敏感大鼠高血压模型中,研究肾脏内源性硫化氢（H2S）体系的变化规律.方法 16只雄性Dah1大鼠采用随机数字表法分为对照组和高盐组（饲料中盐质量分数为80g/kg）,每组8只.实验8周后,测定24 h尿钠、24h尿蛋白、血清肌酐和血清尿素,观察肾脏显微和超微结构改变,敏感硫电极法测定血清和肾组织H2S水平,实时荧光定量聚合酶链反应（RT-PCR）检测肾组织内源性H2S生成关键酶胱硫醚β-合酶（CBS）、胱硫醚γ-裂解酶（CSE）和巯基丙酮酸硫基转移酶（MPST） mRNA表达,Western blot检测肾组织CBS、CSE和MPST蛋白表达.结果 与对照组比较,高盐组Dah1大鼠血压显著升高,肾功能下降,肾脏病变明显,表现为肾小球节段型硬化,肾小动脉管壁增厚,管腔闭塞.高盐组Dah1大鼠肾脏内源性H2S体系显著下调,表现为血清和肾组织H2S水平下降,肾组织CBS、CSE和MPST mRNA表达降低,CBS蛋白表达降低.结论 高盐饮食刺激Dah1大鼠形成高血压及肾脏损害,肾脏内源性H2S/CBS体系下调.Objective To study the change of the renal endogenous hydrogen sulfide （H2 S） pathway in the development of salt-sensitive hypertension in Dah1 rats.Methods Sixteen male Dah1 rats,in accordance with the random number table,were randomly divided into control group and high salt group fed with diet containing 80 g/kg NaCl.After 8 weeks,24 h urine sodium,24 h urinary protein,serum creatinine and serum urea were measured.The microstructural and ultrastructural changes in kidney were observed with light microscope and electronic microscope.The serum and kidney H2S contents were determined by using sulphur-sensitive electrode method.The mRNA levels of cystathionine β-synthase（CBS）,cystathionine γ-lyase（CSE） and mercaptopyruvate sulfurtransferase（MPST） in renal tissue were determined by means of real-time polymerase chain reaction（RT-PCR）.The protein expressions of CBS,CSE and MPST in renal tissue were detected by using Western blot.Results Compared with control group,high salt group rats had a significant rise in blood pressure,declined renal function,damaged renal structure,segmental glomerular sclero sis,small artery wall thickening,and occlusion of the lumen.Moreover,the endogenous H2S pathway in kidney of Dah1rats with high salt diet was downregulated markedly,demonstrated by the decreased serum and kidney H2S content,the reduced renal CBS,CSE and MPST mRNA expressions and CBS protein expression of kidney tissue.Conclusion The endogenous H2S/CBS pathway is downregulated during the development of salt-sensitive hypertension in Dah1 rats.

关 键 词：高盐 高血压 Dah1盐敏感大鼠 硫化氢 

分 类 号：R544.1[医药卫生—心血管疾病]