喜树果超微粉显微特征及含量分析  

Microscopic characteristics and fluorescence spectroscopic of ultra-fine powder in Fructus Camptothecae

作　　者：王晶[1] 廖娟[1] 刘北忠[1] 蔡艳华[2] 张丹[1] 刁奇志[1] 

机构地区：[1]重庆医科大学附属永川医院中心实验室,重庆402160 [2]重庆文理学院材料交叉学科研究中心,重庆402160

出　　处：《中国医院药学杂志》2014年第13期1075-1078,共4页Chinese Journal of Hospital Pharmacy

基　　金：重庆医科大学附属永川医院资助重点项目(编号:YJZD20120006);重庆市卫生局面上项目(编号:2012-2-167)

摘　　要：目的:观察喜树果超微粉的显微特征并测定喜树碱溶出量。方法:气流粉碎制备喜树果超微粉和残渣,显微镜下观察其细胞学特征。在370/430 nm(λex/λem)波长下测定喜树果残渣和超微粉乙醇提取液中CPT含量。结果:镜下残渣细胞结构清晰可见,而超微粉无完整细胞存在,且粒径小于50μm。一定浓度的10-羟基喜树碱能猝灭喜树碱的荧光。喜树果超微粉的乙醇提取液中喜树碱含量有显著性差异(t=4.809,P<0.05)。结论:超微粉中活性成分溶出率高,荧光法测定喜树果药材中喜树碱含量。OBJECTIVE To observe the microscopic characteristics and determine the camptothecin contents of Fructus Camptothecae ultrafine powder. METHODS Fructus Camptothecae were shattered by jet mill and divided to ultrafine and fine powder. Cytological features were visualized using a BXS1 microscope. Using ethanol as solvent, the extracting solution of ultrafine and fine powder were measured fluorescence intensity at λex/λem （370/430 nm） to determine the content of CPT. RESULTS Cellular structure of fine powder was visible while ultrafine powder with a diameter under 50/~m had no complete cells. The fluorescence intensity of CPT was quenched by 10-hydroxy camptothecin in high concentrations, but it did not be interfered by a low concentration of 10-hydroxy camptothecin. The concentration of CPT between ultrafine and fine powder had a statistically significant difference（t = 4. 559,P〈0. 05）. CONCLUSION Micronization can improve dissolution content of CPT. Fluo- rescence method can be used for quality evaluation of CPT in Fructus Camptothecae.

关 键 词：喜树果 超微粉 喜树碱 10-羟基喜树碱 荧光法 

分 类 号：R931.6[医药卫生—生药学]