当归贝母苦参丸对顺铂化疗H22荷瘤小鼠的抑瘤作用及对血清HIF-1α和LDH的影响  

Tumor-inhibitory role of Danggui Beimu Kushen Pills in cisplatin chemotherapy to H22 tumor-bearing mice and effect on serum levels of HIF-1α and LDH

作　　者：闫德祺[1] 刘永琦[1,2,3] 李应东[1] 李豆[1] 蔺兴遥[1] 伍志伟[1] 颜春鲁[1] 

机构地区：[1]甘肃中医学院系统生物学与中医药转化研究所,甘肃兰州730020 [2]甘肃省中药药理与毒理学重点实验室中西医结合基础室,甘肃兰州730020 [3]敦煌医学与转化省部共建教育部重点实验室,甘肃兰州730020

出　　处：《中成药》2014年第7期1351-1355,共5页Chinese Traditional Patent Medicine

基　　金：甘肃省高校基本科研业务费基金资助项目(2009-192-3);国家科技支撑计划项目(2011BAI05B02)

摘　　要：目的探讨当归贝母苦参丸(当归、浙贝母和苦参)对顺铂化疗H22荷瘤小鼠的抑瘤作用及其对血清低氧诱导因子HIF-1α和乳酸脱氢酶(LDH)的影响。方法将雌雄各32只荷H22肝癌移植瘤小鼠随机分为模型组、顺铂组、当归贝母苦参丸组、当归贝母苦参丸与顺铂联合组,另设正常对照组(n=16)。观察各组小鼠一般状况,检测有效体质量变化及抑瘤率、q值、肿瘤指数、肝脏、脾脏、胸腺、和肾脏的器官指数。血常规检测白细胞计数,ELISA检测血清HIF-1α的水平,血生化检测LDH活力。结果当归贝母苦参丸组与联合组的一般状况优于模型组和顺铂组;各治疗组瘤体质量均低于模型组瘤体质量(P<0.05),联合组的抑瘤率高于单用当归贝母苦参丸或顺铂的抑瘤率,联合用药疗效具有相加效应;当归贝母苦参丸组有效体质量增加、脾脏和胸腺指数高于顺铂组(P<0.05);当归贝母苦参丸组和联合组血清HIF-1α水平低于模型组(P<0.05),两组血清LDH活性低于顺铂组(P<0.05)。结论当归贝母苦参丸具有抑瘤作用和对顺铂化疗的增效减毒作用,抑瘤作用可能与降低小鼠血清中HIF-1α水平和降低顺铂引起的LDH活性升高所致的组织损伤。AIM To study tumor-inhibitory role of Danggui Beimu Kushen Pills（ Angelicae sinensis Radix,Fritillariae thunbergii Bulbus,Sophorae flavescentis Radix） in cisplatin chemotherapy to H22 tumor-bearing mice and its effect on serum levels of hypoxia inducible factor 1α（ HIF-1α） and lactate dehydrogenase（ LDH）.METHODS H22 hepatocarcinoma xenograft models were established to be randomly divided into the model group,cisplatin group,Danggui Beimu Kushen Pills group,Danggui Beimu Kushen Pills plus cisplatin group. Normal group was also established. General state of KM mice was observed. Effective changes of body weight,tumor inhibition rate,q value,as well as indexes of tumor,liver,spleen,kidney,and thymus were calculated. Blood routine was done to determine white blood cell count. Serum HIF-1α and LDH were determined by ELISA and biochemistry method,respectively. RESULTS The general state of Danggui Beimu Kushen Pills plus cisplatin group and Danggui Beimu Kushen Pills group was better than that of cisplatin group. The effective body weight of Danggui Beimu Kushen Pills group and Danggui Beimu Kushen Pills plus cisplatin group was higher than that of cisplatin group（ P〈 0. 05）. The mass of tumor in Danggui Beimu Kushen Pills group,cisplatin group,and Danggui Beimu Kushen Pills plus cisplatin group was lighter than that in the model group（ P 〈0. 05）. The tumor inhibiting rate of Danggui Beimu Kushen Pills plus cisplatin group was better than that of both Danggui Beimu Kushen Pills group and cisplatin group. Danggui Beimu Kushen Pills and cisplatin had cooperative effect. The indexes of spleen and thymus in Danggui Beimu Kushen Pills group were higher than those in cisplatin group（ P 〈 0. 05）.The numbers of Neutro-phils and monocyte in Danggui Beimu Kushen Pills group and Danggui Beimu Kushen Pills plus cisplatin group were higher than those in cisplatin group（ P〈 0. 05）. Serum level of HIF-1α in model group was higher than that of Danggui Beimu Kushen Pills group and Danggui Beimu

关 键 词：当归贝母苦参丸 H22肝癌细胞株 抑瘤率 HIF-1Α LDH 

分 类 号：R285.5[医药卫生—中药学]