茵陈蒿汤对A血型抗原免疫小鼠TNF-α水平的影响  

Effects of Yinchenhao decoction on TNF-α level of BALB /c mouse immunized with A blood group antigen

作　　者：李慧[1] 余明明[1] 闫清雅[1] 于艳涛 晁艳[1] 张健媛[1] 刘持翔[1] 石文[1] 周华友[1] 

机构地区：[1]广东省中医院广州中医药大学第二附属医院,广东广州510120 [2]顺德中心血站

出　　处：《中国输血杂志》2014年第6期572-575,共4页Chinese Journal of Blood Transfusion

基　　金：广东省科技计划项目(2010B031600267);广州中医药大学创新基金(2007C072)

摘　　要：目的研究茵陈蒿汤对ABO血型抗原免疫小鼠肿瘤坏死因子-α(TNF-α)分泌水平的影响及TNF-α与抗-A水平变化之间的相关性。方法以人A型红细胞免疫BALB/c小鼠为模型,通过ELISA检测小鼠外周血TNF-α水平变化,应用间接抗球蛋白试验,吸收放散试验检测小鼠血清IgG型抗-A,观察A型红细胞免疫组和茵陈蒿汤治疗组中BALB/c小鼠体内TNF-α水平变化及与IgG型抗-A水平之间的相关性。结果对照组、A型红细胞免疫组和茵陈蒿汤治疗组TNF-α检测结果分别为:(83.50±6.32)ng/L、(135.56±44.14)ng/L和(109.44±12.50)ng/L,组间比较存在统计学差异(P<0.05),组间两两比较P值均<0.05;茵陈蒿汤治疗组有效率为61.11%,有效组和无效组之间TNF-α含量比较无统计学差异;TNF-α水平与抗-A水平之间呈显著性正相关关系(P<0.05)。结论小鼠动物实验提示茵陈蒿汤可抑制TNF-α和ABO血型抗体的分泌,TNF-α水平和ABO血型抗体水平之间的变化呈正相关。Objective To research effects of Yinchenhao decoction on secretion of tumor necrosis factor-α（ TNF-α） in mice immunized with ABO blood group antigens and to investigate the correlation of TNF-α level with the changes of anti-A. Methods Taking BALB/c mice immunized with human erythrocytes of A blood group as a model,the TNF-α in BALB/c mice plasma was detected with ELISA,and the IgG-typed anti-A of BALB/c mice was assayed with indirect antiglobulin test（ IAT） as well as absorption and elution test. The correlation of TNF-α level with the changes of IgG-typed anti-A,in immune group treated with A-type erythrocytes and therapy group treated with Yinchenhao decoction,was investigated. Results TNF-α in control group,A-type erythrocyte immune group and Yinchenhao decoction treatment group were（ 83. 50 ± 6. 32） ng/L,（ 135. 56 ± 44. 14） ng/L and（ 109. 44 ± 12. 50） ng/L,respectively. There existed significant differences between groups（ P 〈0. 05）,and showed all P〈 0. 05 by multiple comparisons. The effective rate in Yinchenhao decoction treatment group was 61. 11%,and there showed no significant differences of TNF-α levels in effective group than in ineffective group. There showed a significant positive correlation between the TNF-α levels and the changes of anti-A（ P〈 0. 05）. Conclusion BALB/c mouse study suggested that Yinchenhao decoction can inhibit the secretion of TNF-α and ABO blood group antibodies,and there showed a significant positive correlation between the levels of TNF-α and the changes of anti-A.

关 键 词：茵陈蒿汤 肿瘤坏死因子-Α 抗-A 抑制 相关性 

分 类 号：R457.11[医药卫生—治疗学]