广东地区临床患者和献血者HCV感染者HCV基因分型比较  

The comparison of HCV subtypes between clinical patients and blood donors in Guangdong Province,China

作　　者：戎霞[1] 郑英[2] 熊华平[1] 许茹[1] 黄杰庭[1] 王敏[1] 黄珂[1] 廖峭[1] 付涌水[1] 

机构地区：[1]广州血液中心,广东广州510095 [2]南方医科大学第三附属医院

出　　处：《中国输血杂志》2014年第6期593-596,共4页Chinese Journal of Blood Transfusion

基　　金：国家自然科学基金(81273145);广东省自然科学基金(S2013010015459);广州市卫生科技重大项目(20141A031001)

摘　　要：目的了解广东地区临床患者和献血者HCV基因型(亚型)分布。方法收集广东地区临床患者HCV RNA阳性标本191(人)份、无偿献血者HCV RNA阳性标本222(人)份,采用逆转录巢式PCR对HCV E1基因扩增并测序;应用MEGA5软件构建分子进化树并对其做基因分型。采用SPSS16.0软件对2组人群中不同基因亚型的分布做比较。结果共检测出HCV-1a、1b、2a、3a、3b、6a和6n等7个基因亚型,其中临床患者分别为1.05%(2/191)、66.49%(127/191)、8.90%(17/191)、2.62%(5/191)、2.62%(5/191)、17.80%(34/191)和0.52%(1/191);献血者分别为0.45%(1/222)、41.44%(92/222)、6.76%(15/222)、8.56%(19/222)、4.95%(11/222)、37.39%(83/222)和0.45%(1/222)。2组人群比较,临床患者1b的比例高于献血者(P<0.01),献血者中3a、6a的比例高于临床患者(P<0.05及P<0.01)。结论广东地区临床患者和献血者中的HCV基因亚型主要是HCV-1b和6a,但HCV-1b、3a和6a亚型在2组人群中所占的比例不同。Objective To investigate the prevalence of HCV subtypes in clinical patients and blood donors in Guangdong province,China. Methods 191 samples of clinical patients and 222 samples of blood donors whose HCV RNA were positive were collected from Guangdong area. HCV E1 gene was amplified by RT-nested PCR and then sequenced on both directions. HCV subtypes were assigned by constructing phylogenetic trees with MEGA5 software. Moreover,SPSS16. 0 software was applied to compare the difference between these two groups. Results Of 191 clinical patients,HCV genotype1a,1b,2a,3a,3b,6a and 6n was 1. 05%（ 2 /191）,66. 49%（ 127 /191）,8. 90%（ 17 /191）,2. 62%（ 5 /191）,2. 62%（ 5 /191）,17. 80%（ 34 /191） and 0. 52%（ 1 /191）,respectively. Of 222 blood donors,HCV genotype 1a,1b,2a,3a,3b,6a and 6n was0. 45%（ 1 /222）,41. 44%（ 92 /222）,6. 76%（ 15 /222）,8. 56%（ 19 /222）,4. 95%（ 11 /222）,37. 39%（ 83 /222） and0. 45%（ 1 /222）,respectively. The proportion of HCV-1b was higher in clinical patients than in blood donors（ P〈 0. 01）,while the proportion of 3a and 6a subtypes were higher in blood donors than in clinical patients（ P〈 0. 05 and〈 0. 01）.Conclusion In Guangdong province,HCV-1b and 6a remained the predominant subtypes in clinical patients and blood donors. The proportion of HCV-1b,3a and 6a subtypes are significantly different between clinical patients and blood donors.

关 键 词：临床患者 献血者 丙型肝炎病毒 基因亚型 广东 

分 类 号：R446.113[医药卫生—诊断学] R512.62[医药卫生—临床医学]