贵州汉族人群人类血小板抗原基因多态性分析  

The analysis of the polymorphism of human platelet antigen genes in Han population,Guizhou,China

作　　者：尹红 杨晋渝[1] 杨芳[1] 曾小菁[1] 钟娜娜[1] 

机构地区：[1]贵阳医学院医学检验学院,贵州贵阳550004 [2]息烽县小寨坝中心卫生院

出　　处：《中国输血杂志》2014年第6期600-602,共3页Chinese Journal of Blood Transfusion

基　　金：贵州省科委资助基金(No.E2009-17)

摘　　要：目的研究贵州地区汉族人群HPA-1～5、15基因多态性分布特点,为本地区HPA免疫性疾病的防治和HPA基因分型库的建立提供参考数据。方法采用PCR-SSP方法对本地区700例汉族人群无血缘关系的健康献血员进行HPA-1～5、15系统基因分型,计算基因型及基因频率,总结出多态性分布特点,并与国内其他地区和国外不同人种进行比较,阐明本地区特点。结果各系统均呈多态性分布。HPA-1和-4接近aa基因型单线分布;HPA-2和-5少量杂合,ab杂合子依次为64和30例,4例-2bb;HPA-3和-15高度杂合,aa基因型依次为242和210例,ab型依次为347和350例,且有较多的bb基因型,依次为111和140例。与其他地区和不同国家人群人种的比较均或多或少存在差异,并随地域和人种的差别而增加。结论贵州地区汉族人群HPA-1～5、15系统基因均呈多态性分布,以HPA-15、-3、-2和-5的a与b基因杂合率较高,对于临床HPA免疫性疾病的防治应给予高度重视。Objective To investigate the characteristics of distribution of HPA-1 ～ 5,15 gene polymorphism of Guizhou Han population,and to provide reference data for the prevention and control of HPA autoimmune disease,and the establishment of the genotyping library in Guiyang. Methods By adopting the method of PCR-SSP to 700 unrelatedhealthy blood donors for HPA-1 ～ 5,15 systems genotyping. The gene frequency and genotype were calculated,and the polymorphism distribution was summed up,and the difference with other populations were compared,in order to clarify the region characteristics.Results All systems were polymorphism distribution. HPA-1 and-4 close to aa single genotype distribution; HPA-2 and-5have a small amount of hybrid,ab heterozygote followed by 64 and 30 cases,there are 4 cases-2 bb; HPA-3 and 15 highly heterozygous,aa genotype respectively 242 and 210,ab in order for 347 and 350,and there are more bb genotype,turn for 111and 140 cases. The differences between other regions and countries increased with regional and ethnic differences. Conclusion The HPA-1 ～ 5,15 system genes of Guizhou province Han population were polymorphism distribution. a and b of HPA-15,3,2 and 5 gene hybrid rate is higher,which should been given more attention in the clinical prevention and control of HPA autoimmune diseases.

关 键 词：贵州地区 汉族 人类血小板抗原 基因多态性 PCR-SSP 

分 类 号：R457.11[医药卫生—治疗学]