山豆根多糖及其硫酸酯体外抗DHV-1感染细胞作用的比较  

Comparison of bush sophora root polysaccharide and its sulfate against DHV-1 infecting cell in vitro

作　　者：陈云[1] 曾玲[1] 熊文[1] 张玲 张清 王德云[1] 刘家国[1] 

机构地区：[1]南京农业大学中兽医学研究室,江苏南京210095 [2]安徽省濉溪兽医院 [3]安徽濉溪动物卫生监督所,安徽淮北235100

出　　处：《南京农业大学学报》2014年第4期117-122,共6页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(31172355);国家公益性行业(农业)科研专项(201303040;201403051);山东省科技发展计划项目(2012GGC15003);江苏高校优势学科建设工程项目(PAPD)

摘　　要：为研制抗1型鸭肝炎病毒(DHV-1)新药成分,用水煎醇沉法提取山豆根多糖(BSRPS),并采用氯磺酸-吡啶法制备其硫酸化产物山豆根多糖硫酸酯(sBSRPS),然后运用体外细胞培养法比较研究BSRPS和sBSRPS抗DHV-1感染鸭胚肝细胞的作用。采用直接荧光免疫法比较分析BSRPS和sBSRPS对DHV-1吸附肝细胞作用的差异,并用实时荧光定量PCR法比较分析了最有效药物浓度的BSRPS和sBSRPS对DHV-1在鸭胚肝细胞上的复制、释放的影响。结果表明:BSRPS和sBSRPS都具有较好的体外抗DHV-1的作用,sBSRPS的效果优于BSRPS。sBSRPS能有效抑制DHV-1的体外复制和释放,BSRPS仅能抑制DHV-1的复制。两者对病毒吸附鸭胚肝细胞没有明显的作用。结论:sBSRPS可以作为抗DVH的新药主要成分之一。The aim of this paper is to research and develop new antiviral drugs ingredients against duck hepatitis virus type 1（DHV-1）.In this paper,the bush sophora root polysaccharide（BSRPS） was extracted by water-extraction and alcohol-precipitation method,and its sulfated product sulfated bush sophora root polysaccharide（sBSRPS） was prepared by chlorosulfonic acid-pyridine method.Antiviral activity against DHV-1 was studied by duck embryonic hepatocytes（DEH） culture in vitro. Direct immunofluorescence method and real-time polymerase chain reaction（real-time PCR） were applied to study the antiviral mechanism of adsorption,replication and release.The results showed that both BSRPS and sBSRPS could exhibite good anti-DHV-1 activities in vitro and sBSRPS performed more effectively than BSRPS. sBSRPS could inhibit both the replication and release of DHV-1 on DEH,while BSRPS only inhibited the replication.Both BSRPS and sBSRPS didn＇t show any influence on the adsorption of DHV-1.Conclusions：sBSRPS would be expected to exploit into the preliminary ingredient of new-type anti-DHV-1 drug.

关 键 词：1型鸭肝炎病毒 山豆根多糖 硫酸化修饰 抗病毒活性 

分 类 号：S853.74[农业科学—临床兽医学]