家蚕感染BmCPV后中肠自噬相关蛋白ATG8的表达变化  

Expressional Variation of Autophagy-related Protein ATG8 in the Midgut of Silkworm( Bombyx mori) During BmCPV Infection

作　　者：吴永鹏[1] 刘立群[1] 朱大鹏[1] 杨晓冰[1] 张敏娟[1] 钱永华[1] 焦锋[1] 

机构地区：[1]西北农林科技大学蚕桑丝绸研究所,陕西杨凌712100

出　　处：《蚕业科学》2014年第3期475-481,共7页ACTA SERICOLOGICA SINICA

基　　金：公益性行业(农业)专项(No.201303057);现代农业产业技术体系建设专项(No.CARS-22);西北农林科技大学基本科研业务专项(No.ZD2012008;XNY2013-27)

摘　　要：家蚕质型多角体病毒（BmCPV）感染家蚕幼虫中肠上皮细胞而引起中肠型脓病,对病毒致病机理的研究有助于制定科学的预防策略。细胞自噬作为机体的一种免疫机制在抵抗微生物的感染中发挥作用。利用免疫组织化学法,检测BmCPV感染家蚕4龄幼虫中肠组织中的细胞自噬相关蛋白ATG8（autophagy-related gene 8）的表达变化：家蚕幼虫在感染BmCPV1-8 h内,中肠ATG8蛋白的表达显著高于正常水平;感染8-35 h,中肠ATP8蛋白的表达下降至正常水平;感染51 h,该蛋白质在中肠的表达又显著升高,至99 h达到高峰,且感染后62 h可以观察到中肠上皮层柱状细胞内有病毒多角体的产生。研究结果显示BmCPV感染家蚕幼虫的过程与中肠细胞自噬存在一定的关系。Bombyx moil cytoplasmic polyhedrovirus （BmCPV） infects epithelial cells of silkworm larval midgut, leading to midgut polyhedrosis. Understanding the mechanism of BmCPV infection can contribute to formulate scientific preven- tion strategies. As an immune mechanism, autophagy plays important role in the resistance of microorganisms. The ex- pressional variation of autophagy-related gene 8 （ATG8） protein in silkworm larval midgut of the 4th instar was assayed using immunohistochemical method. The results showed that the expressional level of ATG8 protein in midgut was sig- nificantly higher than the normal level at 1 h to 8 h after the silkworm larvae were infected by BmCPV, but decreased to background level at 8 h to 35 h post infection. Then the expressional level of ATG8 protein increased sharply again at 51 h and achieved a peak at 99 h. At 62 h post infection, viral polyhedra emerged in the columnar cells of midgut epi- thelial layer. These results suggest that there is certain relationship between autophagy response and BmCPV infection in silkworm larvae.

关 键 词：家蚕质型多角体病毒 家蚕中肠组织 细胞自噬 ATG8蛋白 免疫组织化学法 

分 类 号：S881.2[农业科学—特种经济动物饲养]