低碘环境下氟中毒子代大鼠甲状腺的病理学观察  

Thyroid Histopathology in Offspring of Fluorosis Rat under Low Iodine

作　　者：李冲[1] 娄政驰[2] 陈玲丽[1] 孔令芸 孙相和 黄静[1] 郭东光[1] 宁红梅[1] 王选年[1,3] 葛亚明[1] 

机构地区：[1]河南科技学院,河南新乡453003 [2]新乡医学院第三附属医院,河南新乡453003 [3]新乡学院生物技术研究中心,河南新乡453003

出　　处：《湖北农业科学》2014年第8期1845-1847,1850,共4页Hubei Agricultural Sciences

基　　金：河南省科技厅攻关项目(1221021101150);河南省教育厅自然科学项目(2010B230003)

摘　　要：研究高氟与低碘条件下对子代大鼠甲状腺的影响情况。健康Wistar大鼠(雌∶雄=3∶1)32只随机分为4组。对照组饲喂大鼠标准饲料,饮用去离子水;高氟组饲喂大鼠标准饲料,饮100mg/L氟化钠(NaF)去离子水;低碘组饲喂低碘饲料,饮用去离子水;高氟低碘组饲喂低碘饲料,饮100mg/L氟化钠(NaF)去离子水。模型建立后,生产子代,分别取10、20、30日龄子代大鼠甲状腺称重并进行HE染色。结果表明,与对照组相比,3个试验组的甲状腺出现了不同程度的损伤,但试验组间甲状腺重与体重比值差异不显著。高氟组甲状腺滤泡不完整,且含有细胞碎片;低碘组甲状腺滤泡大小不等,上皮细胞有的呈高柱状;而高氟低碘组的甲状腺滤泡除具有低碘组的特点外,还伴有局灶性细胞坏死。表明碘缺乏情况下氟中毒对大鼠甲状腺的损害更大。The effects of high-fluoride and low-iodine on the thyroid of offspring rats were studied. Thirty-two health Wistar rats were randomly divided into four groups （female：male=3：1） and treated with water, high-fluoride（100mg NaF∕L）, low-io-dine（0.085 8μg∕g） or both high-fluoride （100 mg/L NaF）and low-iodine （0.085 8μg∕g） for 3 months, respectively. The off-spring rats were treated as same. The thyroid gland in 10-day, 20-day and 30-day old offspring rats were taken and stained by HE. The results showed that compared with the control, the thyroid glands in all three treated groups appeared different damage, but the mean values of thyroid weight in the experimental groups were not significantly different. The thyroid follicles in high fluoride group were not complete and contained cellular debris. The thyroid follicles in low iodine group had different sizes and some epithelial cells had high columnar. The thyroid follicles in high fluoride and low iodine group showed focal necrosis besides the characteristics in low iodine group. It is indicated that damage of thyroid gland caused from Fluorosis were much more serious under iodine deficiency.

关 键 词：高氟低碘 子代大鼠 甲状腺 HE染色 

分 类 号：R599.9[医药卫生—内科学]