鹅掌楸叶柄愈伤组织诱导优化  

Optimizing Callus Induction of Liriodendron chinensis Sarg. Petiole

作　　者：吕俊琴[1] 刘中兵[2] 陈玲[2] 

机构地区：[1]武汉生物工程学院生物科学与技术系,武汉430415 [2]武汉生物工程学院园林系,武汉430415

出　　处：《湖北农业科学》2014年第8期1933-1935,共3页Hubei Agricultural Sciences

基　　金：湖北省自然科学基金项目(2009CDZ034);湖北省高等学校青年教师深入企业行动计划项目(XD2012507)

摘　　要：以鹅掌楸(Liriodendron chinensis Sarg.)嫩叶叶柄为外植体,优化了愈伤组织诱导条件。结果表明,叶柄经1 g/L的HgCl2溶液处理9 min,接种至添加2 g/L 50%多菌灵的MS培养基中培养20 d时,成活率最高,达58.8%;愈伤组织诱导的最佳培养基是1/2 MS+0.2 mg/L 2,4-D+2.0 mg/L 6-BA+0.15 mg/L NAA,诱导率达74.37%。To explore the best method of explants disinfection and optimal condition of callus induction, Callus were induced from explants of Liriodendron chinensis petiole. The results showed that when the petiole was treated by 0.1% HgCl2 for 9 min and cultivated in MS medium with 2 g/L 50% carbendazim, Wp has the lowest contamination rate of 41.2%. The optimal formula of callus induction was 1/2 MS＋0.2 mg/L 2,4-D ＋ 2.0 mg/L 6-BA ＋0.15 mg/L NAA with the rate of callus induction of 74.37%.

关 键 词：鹅掌楸(Liriodendron CHINENSIS Sarg ) 叶柄 正交试验 灭菌 愈伤组织诱导 

分 类 号：S792.21[农业科学—林木遗传育种]