草莓花枯病原真菌的PCR检测  

PCR Detection of Rhizoctonia fragariae Husain et W.E.McKeen

作　　者：王振华 张建坤[2] 徐家文 龚国祥 曾宪东 郑剑 邱国强 

机构地区：[1]湖北出入境检验检疫局,武汉430050 [2]武汉理工大学理学院,武汉430070

出　　处：《湖北农业科学》2014年第9期2052-2054,共3页Hubei Agricultural Sciences

基　　金：国家质检总局科技项目(2012IK274)

摘　　要：为了建立简易、快速的草莓花枯病原真菌(Rhizoctonia fragariae)的PCR检测技术,以从美国ATCC购买的标准菌株14691TM为试验材料,通过真菌的通用引物对ITS4和ITS5的PCR扩增,从受感染草莓的根、叶、花、果实4个部位分离的菌株扩增到长度为670 bp的特异性条带,DNA测序证实了这一扩增产物与NCBI的GenBank内草莓花枯病原真菌的相关序列有高度序列同源性。结合草莓根、叶、花、果实的接种及分离、病原菌形态观察及致病性鉴定等结果,确定通用引物PCR扩增技术可以作为草莓花枯病菌检测的基本方法。A standard isolate 14691TMfrom ATTC（U.S.A.） was used to explore an easy and quick PCR detection of Rhizoctonia fragariae Husain et W.E.McKeen. PCR amplifications focusing on ITS4 and ITS5 regions by universal fungal primers were done, DNA bands of 670 bp were obtained from root, leaf, blossom and fruit isolates of infected hosts. Results of DNA sequencing showed that the amplified products had high homogeneity with the related sequence in GenBank, NCBI.According to the results of pathogen inoculation and isolation of root, leaf, blossom and fruit, morphological observation and pathogenicity analysis, the universal primer-PCR was a basic method to detect Rhizoctonia fragariae Husain et W.E.McKeen.

关 键 词：草莓花枯病菌(Rhizoctonia fragariae) 植物检疫 PCR 

分 类 号：S411[农业科学—植物保护]