金钗石斛的快速繁殖研究  

the Rapid Propagation of Dendrobium nobide Lindl.

作　　者：李静婷[1] 牛磊[2] 侯玉杰[3] 

机构地区：[1]平顶山学院资源与环境科学学院,河南平顶山467000 [2]河南城建学院测绘工程学院,河南平顶山467000 [3]河南城建学院生物科学与工程学院,河南平顶山467000

出　　处：《湖北农业科学》2014年第9期2181-2184,共4页Hubei Agricultural Sciences

基　　金：河南省教育厅科技攻关计划项目(12B180020);平顶山市科技攻关计划项目(200622021);平顶山学院高层次人才科研启动项目(2011015G)

摘　　要：以野生金钗石斛(Dendrobium nobide Lindl.)茎段为试验材料,筛选启动培养时的最佳取材部位、最适基本培养基、生根培养基与栽培基质,研究金钗石斛快速繁殖的途径。结果表明,启动培养时用金钗石斛幼茎基部接种,在MS+6.0 mg/L 6-BA+0.2 mg/L NAA+100 mL/L椰乳+25 g/L蔗糖+6.3 g/L琼脂的培养基上效果最好,诱芽率可达93.00%。生根用MS+6.0 mg/L NAA+0.5 mg/L IBA+100 g/L香蕉+2.0 g/L活性炭+25 g/L蔗糖+6.3 g/L琼脂的培养基效果最好,生根率高达95%。最优的组培苗栽培基质是水苔+蛭石(3∶2,V∶V),成活率高达90%。The stems of wild Dendrobium nobile Lindl. were used as materials to select the optimal positions and the preferable medium for induction, root and transplantation. The results showed that the preferable production medium for inducing basal part of young stems was MS＋6.0 mg / L 6-BA＋0.2 mg / L NAA＋100 mL / L coconut milk＋25 g / L cane sugar ＋6.3 g / L agar,with buds induction rate of 93.00%. The preferable medium for rooting was MS＋6.0 mg / L NAA＋0.5 mg / L IBA＋100 g / L banana＋2.0 g / L activated carbon＋25 g / L cane sugar＋6.3 g / L agar, with root induction rate of 95%. The optimal transplantation medium was sphagna＋vermiculite（3∶2,V∶V）, with the survival rate of more than 90%.

关 键 词：金钗石斛(Dendrobium nobide Lindl ) 快速繁殖 培养基 栽培基质 

分 类 号：S567.23[农业科学—中草药栽培] R282[农业科学—作物学]