干露胁迫对刺参体壁非特异性免疫的影响  

Effects of Desiccation on Non-Specific Immune Indices in Sea Cucumber Apostichopus japonicus under Different Temperatures

作　　者：田相利[1] 何瑞鹏 钱圆[1] 臧元奇[1] 董双林[1] 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003

出　　处：《河北渔业》2014年第7期21-26,35,共7页

基　　金：国家"十二五"科技支撑计划项目(2011BAD13B03);山东省杰出青年基金项目(JQ201009);青岛市关键攻关计划项目(12-6-1-6-hy)资助

摘　　要：研究了不同温度（4、8、12、16、20、24℃和温度突变）下干露对刺参体壁非特异性免疫的影响。结果表明，在温度20和24℃条件下干露24 h后，刺参全部死亡，其余处理组刺参成活率为100％。刺参体壁水解酶类和抗氧化指标对于不同温度下干露胁迫具有不同的响应：（1）在本研究的温度范围内，刺参体壁酸性磷酸酶（ACP）、碱性磷酸酶（AKP）活性总体随温度升高而增高，随干露时间的延长而逐渐下降；（2）刺参体壁中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和总抗氧化（T -AOC）活性等抗氧化指标呈现随温度的升高先增强，再逐渐下降的趋势，以16℃为最高。对于干露胁迫时间的响应，抗氧化酶活性等则在低温时（4～16℃）呈现先升高，然后逐渐下降的趋势，在高温（20和24℃）干露胁迫下，则呈现持续下降的趋势。（3）而模拟干法运输的刺参非特异性免疫指标的响应，总体上与12～16℃下干露胁迫相当，而且4～24 h之内，其A K P、SOD活性和T -AOC和MDA数值基本保持稳定。本研究结果表明，刺参干法运输中保持适当的低温可有效降低刺参的胁迫反应，提高刺参运输的成活率。Sea cucumber A postichopus j aponicus with body weight of （50 ± 5）g were exposed to differ-ent desiccation temperatures （4 ℃ ,8 ℃ ,12 ℃ ,16 ℃ ,20 ℃ ,24 ℃ and sudden temperature change） for 0h ,2 h ,4 h ,8 h ,12 h ,24 h and sampled for body wall to study changes of weight loss ratio ,coelomic fluid pH and osmotic pressure and six non - specific immune indices including acid phosphatase （ACP） ,alkaline phosphatase（AKP） ,superoxide dismutase（SOD） ,catalase（CAT） ,the total anti-oxi-dation capability（T -AOC） and MDA contents .The results indicated that all the sea cucumber died at 20 ℃ and 24 ℃ after desiccation for 24 h .The survival rates of the other treatments were 100 .0% . T he hydrolases and antioxidases responded differently to temperature and desiccation .T he activity of ACP and AKP changed positively with temperature change ,but negatively with exposure time .The ac-tivities of SOD ,CAT and T -AOC increased firstly and then decreased gradually＆nbsp;with temperature increase ,with the maximum at 16 ℃ .And they increased firstly and decreased gradu-ally at lower temperatures（4~16 ℃） ,but dropped continually at higher temperatures（20~24 ℃）with the time .Under sudden desiccation temperature changes ,the non -specific immune indices of sea cu-cumber changed similarly with that at 12 and 16 ℃ generally .Within 4~24 h ,activities of AKP and SOD ,T -AOC and MDA contents almost kept stable .It can be concluded that it’s beneficial for im-munity of sea cucumber to keep appropriate low temperature during no -water transportation .

关 键 词：刺参 温度 干露 非特异性免疫 

分 类 号：S917.4[农业科学—水产科学]