地高辛抗血清对缺氧损伤心肌组织内源性洋地黄样因子和心肌细胞膜ATP酶活性的影响(英文)  

Effect of anti-digoxin antiserum on endogenous digitalis-like factorand membrane ATPase activity in hypoxic injury myocardium

作　　者：柯永胜[1] 杨浩[1] 杨霆[1] 程光华[1] 汪佑霞[1] 

机构地区：[1]皖南医学院弋矶山医院,芜湖241001

出　　处：《中国临床药理学与治疗学》2000年第1期10-13,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：ProjectsupportedbytheFoundationofAnhuiEducationCommittee№99jl0219

摘　　要：目的评价地高辛抗血清对心肌缺氧损伤的保护作用与机制。方法制备心肌组织缺氧损伤模型 ,观察不同剂量的地高辛抗血清对缺氧损伤心肌组织内源性洋地黄样因子水平和心肌细胞膜ATP酶活性的影响。结果缺氧损伤可使心肌组织内源性洋地黄样因子水平明显升高 ,心肌细胞膜ATP酶活性明显下降 ;地高辛抗血清能明显拮抗缺氧对心肌细胞膜ATP酶活性的抑制作用 ,使酶活性得到恢复。结论缺氧损伤所致心肌组织内源性洋地黄样因子水平升高是缺氧介导心肌损伤的分子生物学基础 ,地高辛抗血清通过拮抗内源性洋地黄样因子的作用 ,减轻缺氧所致心肌损伤 。Aim To evaluate the protective effect of anti_digoxin antiserum on hypoxic injury myocardium and its mechanism. Methods It was observed that different concentration anti_digoxin antiserum effect on endogenous digitalis_like factor and cell membrane ATPase activity in hypoxic myocardium model. Results The level of endogenous digitalis_like factor was remarkably higher, cell membrane ATPase activity were remarkably lower in hypoxic group than those of normal group; anti_digoxin antiserum can resume membrane ATPase activity.Conclusion Rise of endogenous digitalis_like factor was basic of molecular biology of myocardial damage during myocardial hypoxia. Anti_digoxin antiserum has lightened myocardial injury and has protective effect on hypoxic myocardium by against effect of endogenous digitalis_like factor.

关 键 词：地高辛抗血清 ATP酶 心肌缺氧 内源性洋地黄样因子 

分 类 号：R972.1[医药卫生—药品] R542.2[医药卫生—药学]