脾切除对肝硬化门静脉高压症大鼠免疫功能的影响及意义  

Effects and Significance of Splenectomy on the Immunity of Rats with Portal Hypertension

作　　者：余灵祥[1] 张绍庚[1] 郭晓东[2] 肖朝辉[1] 凌海慧[3] 赵德希[1] 吴建中[1] 张培瑞[1] 李志伟[1] 

机构地区：[1]解放军第302医院肝胆外科一中心,北京100039 [2]解放军第302医院病理诊断与研究中心,北京100039 [3]解放军第302医院药学研究中心,北京100039

出　　处：《现代生物医学进展》2014年第23期4417-4419,4433,共4页Progress in Modern Biomedicine

基　　金：北京市科技计划首都临床特色应用研究专项(Z131100004013041);解放军第302医院院内课题(YNKT2013018)

摘　　要：目的:对肝硬化门静脉高压症大鼠行脾切除手术,观察术后大鼠免疫系统功能的变化情况,探讨脾切除术对免疫系统的影响,为临床治疗提供理论基础。方法:选取SD大鼠45只建立PHT模型,随机分为PHT组、脾全切组及部分脾切除组,每组各15只。另选取10只健康大鼠为对照组。分别对四组大鼠行不同的脾切除方案,术后检测四组大鼠的血常规、免疫球蛋白含量、T淋巴细胞亚群的变化及组织病理学染色结果等,分析各指标的意义。结果:四组大鼠血常规检测结果显示,PHT组大鼠WBC高于对照组,PHTTS组高于PHT组,PHTSS组高于PHTTS组,差异显著且具有统计学意义(P<0.05)。四组大鼠血浆中IgG含量差异显著(P<0.05);IgA、IgM含量无显著性差异(P>0.05)。四组大鼠CD4+、CD8+含量差异显著(P<0.05);CD29+含量无显著性差异(P>0.05)。结论:脾脏直接参于细胞介导免疫调节,脾切除可降低门脉压力,消除脾亢,有利于肝硬化门脉高压症的缓解。Objective： To discuss the effects and significance of splenectomy on the immunity of rats with portal hypertension so as to make a reference for the clinical surgery. Methods： Forty-five SD rats were selected to make the PHT model and randomly divided into the three groups which referred to the PHT group, the PHTTS group that taking the total splenectomy surgery and the PHTSS group that undergoing the partial splenectomy surgery. Another fifteen healthy rats were chosen as the control group. Then the detection of the blood, the immunity, the T lymphocyte subsets and the pathological changes of the rats were observed and analyzed. Results： There was statistically significant difference of blood routine of rats between the four groups （P〈0.05）; There was statistically significant difference oflgG in the plasma of rats between the four groups （P〈0.05）; There was no statistically significant difference about the IgA and IgM （P〉0.05）; There were statistically significant differences of CD4＋ and CD8＋ of rats between the four groups （P〈0.05）; There was no statistically significant difference about CD29＋ of rats between the four groups （P〉0.05）. Conclusion： It is indicated that the splenectomy could reduce the portal pressure, eliminate the port and relieve the cirrhotic portal hypertension.

关 键 词：肝硬化门静脉高压 脾切除术 免疫功能 

分 类 号：Q95-3[生物学—动物学]