白藜芦醇通过降低galectin-3的表达抑制宫颈癌SiHa细胞的增殖并促进其凋亡  

Resveratrol Inhibits the Proliferation and Promotes the Apoptosis of SiHa Cells by Reducing Galectin-3 Expression

作　　者：王恒[1] 李亚玲[1] 代维栋[1] 王萍[1] 李江鹏[1] 

机构地区：[1]西安市第四医院妇产科,陕西西安710004

出　　处：《现代生物医学进展》2014年第23期4451-4454,共4页Progress in Modern Biomedicine

基　　金：陕西省科学技术研究发展计划项目(2013K12-03-22)

摘　　要：目的:探讨白藜芦醇对人宫颈癌SiHa细胞增殖和凋亡的影响及galectin-3在其中的作用。方法:以体外培养的人宫颈癌SiHa细胞为研究对象,实验分为对照组、白藜芦醇处理组及[白藜芦醇+galectin-3(5、10、20μg/mL)]共5组,分别给予生理盐水、300μmol/L白藜芦醇及[300μmol/L白藜芦醇+Galectin-3(5、10、20μg/mL)]处理。48小时后,分别收集各组细胞,采用MTT法检测细胞的增殖情况,Caspase-3活性试剂盒测定细胞的凋亡情况,Western Blot技术测定细胞内galectin-3的蛋白表达水平。结果:300μmol/L的白藜芦醇明显抑制SiHa细胞的增殖,并显著增加其凋亡水平,同时减少了细胞内galectin-3的蛋白表达。在白藜芦醇处理的同时,给予5、10及20μg/mL的Galectin-3,随着Galectin-3蛋白浓度的增加,SiHa细胞的增殖抑制率逐渐降低,凋亡水平也逐渐下降,但是VEGF-R3受体的磷酸化水平却逐渐升高。结论:白藜芦醇通过降低galectin-3的表达抑制宫颈癌SiHa细胞的增殖并促进其凋亡。Objective： To observe the effect of resveratrol on the proliferation and apoptosis of SiHa cells, and explore the related role of galectin. Methods： Human cervical cancer cell line SiHa was cultured in vitro, the experiment was divided into 5 groups as follows： control group, resveratrol and [resveratrol＋galectin-3（5 μg/mL, 10 μg/mL and 20 μg/mL）]. They were given normal saline,resveratrol and [resveratrol＋galectin（5 μg/mL, 10 μg/mL and 20 μg/mL）], respectively. 48 hours later, the cells were collected. Cell proliferation was detected by MTT, cell apoptosis was determined by Caspase-3 activity assay kit, the galectin-3 expression was evaluated by Western Blot. Results： Resveratrol（300 μmol/L） obviously inhibited the proliferation of SiHa cells, significantly increased the apoptosis level, and markedly reduced galectin-3 expression. SiHa cells were given different concentrations of galectin-3（5, 10, 20μg/mL） along with resveratrol（300 μmol/L）. With the increase of galectin-3 concentration, the proliferation of SiHa cells gradually increased and the apoptosis level of SiHa cells gradually reduced, however, the phosphorylation level of VEGF-R3 gradually increased.Conclusion： Resveratrol could inhibit the proliferation and promote the apoptosis of SiHa cells via reducing the galectin-3 expression.

关 键 词：白藜芦醇 宫颈癌 半乳糖凝集素-3 增殖 凋亡 

分 类 号：R711.74[医药卫生—妇产科学]