全反式维甲酸和阿糖胞苷对HL-60细胞端粒酶活性的影响  

Effect of the all trans retinoic acid and Ara-c on the telomerase activity of HL-60 cells

作　　者：吴向华[1] 叶开和[1] 胡爱群[2] 

机构地区：[1]暨南大学医学院,广州510630 [2]皖南医学院,芜湖241001

出　　处：《中国临床药理学与治疗学》2000年第1期47-49,共3页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的探讨全反式维甲酸 (alltrans_retinoicacid,ATRA )和阿糖胞苷 (Ara_c)诱导人早幼粒细胞白血病细胞凋亡和分化的机制。方法采用PCR_ELISA方法观察ATRA和Ara_c对HL_60细胞端粒酶活性的影响。结果ATRA和Ara_c在诱导HL_60细胞凋亡和分化过程中均可使端粒酶活性减低。结论ATRA和Ara_c抑制HK_60细胞端粒酶的活性 。Aim To investigate the possible mechanism of human promyelocytic leukemia cells differentiation and apoptosis induced by all trans_retinoic acid (ATRA) and Ara_c. Methods The effects of ATRA and Ara_c on the tolomerase activity of HL_60 cells were detected by PCR_ELISA. Results The tolomerase activity of HL_60 cells declined during the course of differentiation and apoptosis induced by ATRA and Ara_c. Conclusion These findings suggest that ATRA and Ara_c can inhibit tolomerase activity of HL_60 cells. It may be one of the important mechanisms of human promyelocytic leukemia cells differentiation and apoptosis induced by ATRA and Ara_c.

关 键 词：HL-60细胞 端粒酶 全反式维甲酸 阿糖胞苷 

分 类 号：R979.1[医药卫生—药品]