正交试验优选桑籽中总生物碱、总黄酮及粗多糖的提取工艺  

Optimization of Extraction Process for Total Alkaloids,Total Flavonoids,Polysaccharides from Mulberry Seeds by Orthogonal Test

作　　者：吕志强[1] 李乔[2] 刘洪玲[1] 田景振[3] 

机构地区：[1]青岛大学附属医院药剂科,山东青岛266000 [2]青岛酒店管理职业技术学院,山东青岛266000 [3]山东中医药大学药学院,济南250355

出　　处：《中国实验方剂学杂志》2014年第14期27-30,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山东省教育厅科研发展计划项目(J12LM58)

摘　　要：目的:优选桑籽中总生物碱、总黄酮及粗多糖的提取工艺。方法:将桑籽脱脂后,以总生物碱、总黄酮及粗多糖提取量的综合评分为指标,在单因素试验基础上,通过正交试验考察提取时间、乙醇体积分数及盐酸摩尔浓度对提取工艺的影响。总黄酮、总生物碱及粗多糖的含量测定依次采用Al(NO3)3法、雷氏盐比色法及蒽酮-硫酸法。结果:最佳提取工艺为提取时间2 h,乙醇体积分数50%,盐酸摩尔浓度0.03 mol·L-1;总生物碱、总黄酮及粗多糖平均提取量分别为23.15,0.57,0.51 mg·g-1。结论:该工艺稳定可行,提取效率高,适用于工业化生产,为桑籽资源的开发利用提供参考。Objective： To optimize extraction process of total alkaloids, total flavonoids and polysaccharides from mulberry seeds. Method： After mulberry seeds was defatted, with composite score of extracting amounts of total alkaloids, total flavonoids and polysaccharides as indicators, on the basis of single factor tests, orthogonal design was adopted to optimize extraction process by taking extracting time, ethanol concentration, hydrochloric acid concentration as factors. Contents of total flavonoids, total alkaloids and polysaccharides were determined by Al （NO3 ）3, reinecke salt colorimetry and anthrone-sulfuric acid method, respectively. Result： Optimum extraction conditions were as follows： extracting time 2 h, ethanol concentration 50% molar concentration of hydrochloric acid 0. 03 mol· L^-1 , ; average extracting amounts of total alkaloids, total flavonoids and polysaccharides were 23.15, 0.57, 0.51 mg ·g^-1 Conclusion： This optimized extraction process was stable, feasible and suitable for industrial production with high extraction efficiency, it could provide a reference for development and utilization of mulberry seeds resources.

关 键 词：桑籽 总生物碱 总黄酮 多糖类 正交试验 雷氏盐比色法 

分 类 号：R283.6[医药卫生—中药学]