Plackett-Burman设计结合响应面法优化天麻效应成分的提取工艺  

Optimization of Extraction Process for Active Ingredients from Gastrodiae Rhizoma by Plackett-Burman Design and Response Surface Analysis

作　　者：艾志福[1] 伍振峰[1] 张帅杰[1] 韩飞[1] 万晶[1] 

机构地区：[1]江西中医药大学,南昌330004

出　　处：《中国实验方剂学杂志》2014年第14期34-37,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江西省卫生厅中医药科研项目(2012A021)

摘　　要：目的:优选天麻效应成分的提取工艺。方法:采用回流提取法,以天麻素提取率为指标,采用Plackett-Burman设计筛选天麻效应成分的显著性影响因素,通过Box-Behnken设计对影响提取工艺的显著性因素进行优化,试验数据进行多元线性回归和二项式拟合,应用效应面法对最佳工艺进行预测分析。运用HPLC测定天麻素含量,流动相乙腈-0.05%磷酸溶液(3∶97),检测波长220 nm。结果:加液量、提取时间和提取次数对效应成分的提取效率影响较大;二项式方程拟合度较好,预测结果较为准确,最佳工艺条件为加液量10倍,提取时间120 min,提取数3次;天麻素提取率1.392%,与预测值1.397%偏差较小。结论:采用Plackett-Burman设计结合响应面法优选天麻效应成分的提取工艺稳定可行,预测性较好,为天麻的资源开发提供参考。Objective： To optimize extraction process of active ingredients from Gastrodiae Rhizoma in Dachuanxiong formula. Method： Active ingredients from Gastrodiae Rhizoma was extracted by reflux, with yield of gastrodine as index, some factors affecting index were firstly evaluated by Plackett-Burman design, then extraction conditions were optimized by Box-Behnken design, experimental date were imitated by multiple regression and second-order polynomial equation, response surface analysis was adopted to predict optimum process. HPLC was employed to determine the content of gastrodine with mobile phase of acetonitrile-0.05% phosphoric acid solution （3：97） and detection wavelength at 220 nm. Result： Solvent volume, extracting times and time had significant effects on yield of active ingredients; binomial equation fitted well with good predictability, optimal extraction process was as follows： extracted thrice with solid-lipuid ratio of 1： 10, 120 min for each time; yield of gastrodine was 1. 392% , whose deviation was small by comparing with the predicted value of 1.397%. Conclusion： Extraction process of active ingredients from Gastrodiae Rhizoma optimized by combination of Plackett-Burman design and response surface analysis was stable and feasible with goodpredictability, which could serve as a valuable reference for resource development of Gastrodiae Rhizoma.

关 键 词：Plackett—Burman设计 Box—Behnken设计-响应面法 天麻 天麻素 显著性分析 

分 类 号：R283.6[医药卫生—中药学]