暑热宁口服液中百秋李醇、广藿香酮、麝香草酚、香荆芥酚GC测定及条件的优化  

Heat Better Oral Liquid from Best Autumn Lee Alcohol,Ketone of Patchouli,Thymol,Sweet Fineleaf Schizonepeta Herb Phenol Determination of GC Conditions Optimization

作　　者：李凤丽[1,2] 高文远[3] 李进[1] 陈涛[1] 王进博[1] 

机构地区：[1]天津中医药大学第一附属医院,天津300193 [2]天津中医药大学,天津300193 [3]天津大学,天津300193

出　　处：《中国实验方剂学杂志》2014年第14期65-68,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2010ZX09102-201)

摘　　要：目的：建立并优化暑热宁口服液中百秋李醇、广藿香酮、麝香草酚、香荆芥酚的毛细管气相色谱法测定条件。方法：HP-5弹性石英毛细管柱（0．25μm×0．32mm×30m），进样口温度220℃，FID检测器温度220℃，程序升温，起始温度110℃，保持10min，以25℃程序升温至230℃，保持9．2min，分流比10：1，进样量1μL。结果：百秋李醇、广藿香酮、麝香草酚、香荆芥酚在1．109～0．1109，0．504～0．0504，0．987～0．0987，0．605～0．0605g·L^-1与峰面积线性关系良好，线性关系为Y百秋李醇=1653．05X-1．46（r=0．9998），Y广藿香酮=1207．45X-16．10（r=0．9997），Y麝香草酚=1691．68X-18．06（r=0．9995），Y香荆芥酚=1547．18X＋5．55（r=0．9996），加样回收率（n：9）为99．9％～103．7％，98．4％～104．4％，96．5％～101．4％，97．2％～102．6％，RSD分别为1．9％，2．3％，2．5％，2．2％。结论：优化后的测定方法分离度较好，阴性无干扰，操作简便，结果稳定可行。Objective： Optimization and to establish the heat better oral liquid from best autumn lee alcohol, ketone of patchouli, thymol, sweet fineleaf schizonepeta.herb phenol of capillary gas chromatography determination conditions. Method： The chromatographic column- HP-5 （0. 25 μm × 0. 32 mm ×30 m） elastic quartz capillary column; injection port temperature 220 ℃, FID detector temperature 220 ℃, temperature programmed, starting temperature 110 % , keep for 10 min, at 25 ℃ to 230 ℃ , temperature programmed to keep 9.2 min, diversion ratio 10： 1, including 1 L sample quantity. Result： The autumn lee alcohol, ketone of patchouli, thymol, sweet fineleaf schizonepeta herb phenol concentration in sample quantity is 1. 109-0. 110 9, 0. 504-0. 050 4, 0. 987-0. 098 7, 0. 605-0. 060 5 g·L^-1 and good linear relationship between peak area, linear relationship is Y=1 653.05X-1.46 （r=0.999 8）, Y=1 207.45X-16.10 （r=0.9997）, Y=1 691.68X- 18.06 （r=0.9995）, Y=1 547.18X＋5.55 （r=0.999 6） sample recovery rate was 99. 9%-103.7% （n =9）98.4% -104.4%, 104.4%-101.4% and 101.4%-97.2%, RSD was 1.9%, 2.3%, 2.5% and 1.9% respectively. Conclusion： The optimized method has good separating degree, negative without interference, easy operation, stable and feasible.

关 键 词：毛细管气相色谱法 暑热宁口服液 百秋李醇 广藿香酮 麝香草酚 香荆芥酚 测定条件优化 

分 类 号：R284.1[医药卫生—中药学]