SPME-GC-MS法研究竹叶柴胡和北柴胡挥发性成分差异  

Different Analysis of Essential Oil from Bupleurum marginatum and Bupleurum chinese by SPME-GC-MS

作　　者：王砚[1,2] 王书林[1] 

机构地区：[1]成都中医药大学药学院,成都611137 [2]四川省食品药品检验检测院,成都611731

出　　处：《中国实验方剂学杂志》2014年第14期104-108,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家星火计划项目(2010GA810056)

摘　　要：目的:对竹叶柴胡和北柴胡进行挥发性成分的比较和分析,指出竹叶柴胡和北柴胡的差异性和共同性。方法:利用固相微萃取技术(SPME)提取,采用气质联用(GC-MS)进行分离和鉴定。结果:共鉴定出竹叶柴胡48个成分,北柴胡21个成分,其中有10个化合物为共有峰,相对质量分数最高的均为n-十六烷酸。结论:SPME-GC-MS能全面快速分析柴胡挥发油成分,并作为柴胡药材质量评估和基源鉴定的科学手段。Objective： To find the difference and the identity through analyzing the essential oil of Bupleurum marginatum and B. chinese. Method： The essential oil components of B. marginatum and B. chinese were extracted by solid phase micro extraction （SPME） and analyzed by GC-MS. Result： Forty-eight compounds were identified from B. marginatum, and 21 from B. Chinese. Ten common compounds were found and n-hexadecanoic acid was the main compound of highest relative content in both of B. marginatum and B. chinense. Conclusion： The information about the constituents of essential oil can be obtained entirely and quickly by SPME- GC-MS which is the scientific method of quality assessment and origin identification.

关 键 词：竹叶柴胡 北柴胡 挥发油 固相微萃取-气质联用 n-十六烷酸 

分 类 号：R284.1[医药卫生—中药学]