离子对液相色谱法测定柴枳四逆散中辛弗林的含量  

Determination of synephrine in Caizhi Sini Powder by Ion-Pair HPLC

作　　者：邱红[1] 胡筱梅[1] 

机构地区：[1]湖北医药学院附属东风医院检验科,湖北十堰442008

出　　处：《现代仪器与医疗》2014年第4期69-71,共3页Modern Instruments & Medical Treatment

摘　　要：目的：建立测定柴枳四逆散中辛弗林含量的方法。方法 ：采用高效液相色谱法,色谱条件为Fortis C18色谱柱（4.6×250mm,5μm）,流动相为甲醇-磷酸二氢钾溶液（43：57）,流速为1.0ml·min-1,柱温：30℃,检测波长为275nm。结果：辛弗林在1.82～36.40μg·ml-1（r=0.9999）浓度范围内有良好的线性关系,平均加样回收率为98.0%,RSD为1.50%。结论：本方法准确,重复性好,可用于柴枳四逆散中辛弗林的测定。Objective：To establish a method for the determination of synephrine in Caizhi Sini Powder.Method：The RP-HPLC system consisting the Fortis C18（4.6×250mm, 5μm）column, the mobile phase consisted of MeOH -KH2PO4（43： 57）, the lfow rate was 1.0ml·min-1, the column temperature was 30℃,The UV detector was set at 275nm.Results：The linear response range was 1.82~36.40μg·ml-1（r=0.9999）.The average recovery of schisandrin was 98.0%, RSD was 1.50%. Conclusions：The assay demonstrated that the method was simple and accurate, repeatable, it can be applied in determination of schisandrin in synephrine in Caizhi Sini Powder.

关 键 词：辛弗林 柴枳四逆散 离子对高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]