黄芪总提取物抗衰老作用的实验研究  

Anti-oxidative effect of total extract of astragalus(TEA) on aging mice

作　　者：雷红[1] 王斌[2] 李卫平[3] 杨雁[3] 周爱武[3] 陈敏珠[3] 

机构地区：[1]安徽省卫生干部进修学校,合肥230061 [2]北京协和医院风湿免疫学 [3]安徽医科大学临床药理研究所

出　　处：《中国临床药理学与治疗学》2000年第4期298-302,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的 研究黄芪总提取物 (totalextractofastragalus,TEA)延缓衰老的作用机理。方法采用小鼠D_半乳糖 (D_galactose,D_gal)人工衰老模型和自然衰老模型(17mon),从氧自由基代谢方面进行研究。结果TEA(40mg·kg-1·d-1ig×10wk)可明显降低D_gal衰老小鼠过高的MDA含量 ,上调其过低的抗氧化酶 (GSHPX、Mn_SOD)活性和GSH/GSSG比值。TEA(40mg·kg-1·d -1ig×3mon)对17mon小鼠亦有类似作用。结论TEA对D_gal衰老小鼠和由中年期(14mon)进入老前期 (17mon)小鼠的自然衰老进程有明显的延缓作用 。Aim To study the mechanism of the anti-aging effect of total extract of astragalus(TEA) in mice. Methods MDA content, the activities of Mn-SOD and GSHpx and the reduced to oxidized glutathione (GSH/GSSG) ratio in mitochondria of D-galactose(D-gal) treated mice and 17-month-old mice were measured. Results Treatment with TEA(40 mg·kg-1·d-1 ig) for 10 wk would lower the content of MDA and restore activities of Mn-SOD,GSHpx and GSH/GSSG ratio in mitochondria of D-gal treated mice.Treatment with TEA (40 mg·kg-1 ·d-1 ig) for 3 mon had the same effect on 17-month-old mice.Conclusion TEA has anti-aging effect on D-gal treated mice and 17-month-old mice significantly, probably being related to its anti-oxidative effect.

关 键 词：黄芪总提取物 抗衰老 D-半乳糖 脂质过氧化物 谷胱甘肽过氧化物酶 锰-超氧化物歧化酶谷胱甘肽 

分 类 号：R285.5[医药卫生—中药学]