黑龙江省野生秋子梨群体遗传结构的荧光AFLP分析  

Genetic Structure Analyses Based on Fluorescent-AFLP Markers of Wild Populations of Pyrus ussuriensis Maxim.in Heilongjiang Province

作　　者：安萌萌[1] 刘畅[2] 何天明[3] 张艳敏[1] 冀晓昊[1] 王艳廷[1] 李敏[1] 王传增[4] 陈学森[1] 

机构地区：[1]山东农业大学园艺科学与工程学院/作物生物学国家重点实验室,泰安271018 [2]黑龙江省农业科学院牡丹江分院,牡丹江157041 [3]新疆农业大学林学与园艺学院,乌鲁木齐830052 [4]山东省果树研究所,泰安271000

出　　处：《植物遗传资源学报》2014年第4期728-733,共6页Journal of Plant Genetic Resources

基　　金：国家自然科学基金项目(31171932);国家重点基础研究发展计划课题(2011CB100606)

摘　　要：以来自黑龙江省海林市、东宁县和孙吴县3个居群的90个野生秋子梨株系为试材,利用荧光AFLP标记技术对野生秋子梨群体遗传多样性及遗传结构进行了分析,旨在为野生秋子梨种质资源的保护和利用提供科学依据。结果表明:野生秋子梨3个居群种群水平多态性位点百分比(P)为82.95%,Nei's基因多样度(H)为0.1467,Shannon信息指数(I)为0.2397,野生秋子梨遗传多样性水平较低;3个居群的多态性位点百分比、基因多样度及Shannon信息指数比较,东宁居群>海林居群>孙吴居群,居群水平上东宁居群的遗传多样性较丰富;基因分化系数(GST=0.1008)显示,野生秋子梨的遗传变异主要发生在居群内;野生秋子梨居群间存在较大的基因流动(Nm=4.4603),说明3个野生秋子梨居群间存在着较频繁的基因交流;UPGMA聚类分析结果表明,3个居群是相对独立的孟德尔群体,且海林居群和东宁居群的遗传关系较近。3个居群中东宁居群遗传多样性最丰富,是进行原生境保护优先考虑的居群。Using fluorescent AFLP marker, we analyzed population genetic diversity wild Pyrus ussuriensis to provide scientific foundation for wild Pyrus ussuriensis germplasm and genetic structure of conservation and utiliza- tion. 90 accessions from Hailin, Dongning, and Sunwu country of Heilongjiang province were selected as the research materials. The results showed that tile percentage of polymorphic loci （P） was 82.95% at species level, Nei＇s gene diversity （H）was 0. 1467, and Shannon information index （I） was 0. 2397. And the genetic diversity of wild Pyrus ussuriensis was low. The polymorphic loci （P）, Nei＇s gene diversity （H）, and Shannon information index （I） at population level were sequenced from high to low as Dongning population 〉 Hailin popuhion 〉 Sunwu population, which meaned that genetic diversity of Dongning population was the richest in the three populations. The genetic dif- ferentiation coefficient （ Gsr = 0. 1008 ） of the three populations showed that genetic variation mainly occurred within populations. There was higher level of gene flow among the three populations （ Nm = 4. 4603 ） which indicated that there were frequent gene exchange among populations. The UPGMA cluster analysis indicated that the three popula- tions were Mendelian relative independent population, and the closer genetic relationship existed between Hailin andDongning population. Dongning population had the most abundant genetic diversity and also was the preferential population for the implementation of in-situ conservation strategies.

关 键 词：野生秋子梨 荧光AFLP 遗传多样性 群体遗传结构 

分 类 号：S661.2[农业科学—果树学]