CD154与CD4＋CD25＋FoxP3＋调节性T细胞在强直性脊柱炎患者外周血中的表达及意义  

The expression and clinical value of CD40 ligand（CD154） and CD4＋CD25＋FoxP3＋ regulatory T cells in patients with ankylosing spondylitis

作　　者：李秀娟[1] 黄胜起[1] 王阿妮[3] 李时英[1] 陈卫光 韦峰[2] 

机构地区：[1]中山大学附属江门市中心医院检验科,广东省江门529000 [2]中山大学附属江门市中心医院肾内科,广东省江门529000 [3]中山大学附属第五医院心内科 [4]江门市新会区妇幼保健医院检验科

出　　处：《中国基层医药》2014年第13期1942-1944,共3页Chinese Journal of Primary Medicine and Pharmacy

基　　金：广东省医学科研基金资助项目（B2009247）

摘　　要：目的 探讨强直性脊柱炎（AS）患者外周血淋巴细胞中CD154及CD4＋CD225＋FoxP3＋调节性T细胞的表达水平及其意义.方法 采用流式细胞术检测30例AS（AS组）和20例健康人（对照组）外周血淋巴细胞中CD154、CD4＋CD25＋FoxP3＋调节性T细胞及人类白细胞抗原-B27 （HLA-B27）,并分析CD154及CD4＋CD25＋FoxP3＋调节性T细胞与AS活动性指标的相关性.结果 AS组外周血淋巴细胞中CD154表达水平显著高于对照组[（4.38±1.64）％比（1.92±1.13）％（t=5.836,P＜0.05）],CD4＋CD25＋FoxP3＋调节性T细胞表达水平明显低于对照组[（3.87±1.11）％比（6.30±1.46）％（t=6.254,P＜0.05）].AS组外周血淋巴细胞中CD154表达水平与CD4＋CD25＋FoxP3＋调节性T细胞表达水平呈负相关（r=-0.539,P＜0.05）.外周血CD154及CD4＋CD25＋high调节性T细胞表达与HLA-B27平均荧光强度及ESR、CRP无明显相关性（P＞0.05）.结论 AS患者外周血CD154、CD4＋CD25＋FoxP3＋调节性T细胞表达异常,CD154、CD4＋CD25＋FoxP3＋调节性T细胞可能共同参与了AS的免疫发病过程.Objective To investigate the levels and clinical value of the peripheral blood CD154 and CD4＋CD25＋FoxP3＋ regulatory T cells in patients with ankylosing spondylitis （AS）.Methods 20 patients with AS and 20 healthy donors were studied.Flow cytometry was used to analyze the levels of the peripheral blood CD154,CD4＋CD25＋FoxP3＋ regulatory T cells and HLA-B27.And T-test,Spearman correlation analysis were used in statistical analysis.Results The level of the peripheral blood CD154 was significantly higher in AS patients than that in normal controls [（4.38 ± 1.64） % vs.（1.92 ± 1.13） % （t =5.836,P 〈 0.05）],and the level of the peripheral blood CD4＋CD25＋FoxP3＋ regulatory T cells was lower in AS patients than that in normal controls [（3.87 ± 1.11） % vs.（6.30 ± 1.46） % （t =6.254,P 〈 0.05）].There was negative correlation between the peripheral blood CD154 and CD4＋CD25＋FoxP3＋ regulatory T cells in AS （r =-0.539,P 〈 0.05）.No relationship of the peripheral blood CD154,CD4＋CD25＋FoxP3＋ regulatory T cells with laboratory parameters were found in AS.Conclusion The levels of the peripheral blood CD154 and CD4＋CD225＋FoxP3＋ regulatory T cells in AS are abnormal,and may be involved in the immunological pathogenesis of AS.

关 键 词：CD40配体 T淋巴细胞 调节性 脊柱炎 强直性 

分 类 号：R593.23[医药卫生—内科学]