胶原海绵对创面愈合的影响  

The impact of collagen sponge on wound healing

作　　者：乐音子[1] 陈灵[1] 任伟业 郭檬檬[1] 曹东阳[1] 卞卫和[3] 魏玲 姚昶[3] 

机构地区：[1]南京中医药大学,江苏省南京210029 [2]无锡贝迪生物工程有限公司 [3]江苏省中医院乳腺外科

出　　处：《中国基层医药》2014年第13期1954-1956,共3页Chinese Journal of Primary Medicine and Pharmacy

基　　金：江苏省自然科学基金资助项目（BK2010598）;江苏省中医药领军人才基金项目（LJ2009002）

摘　　要：目的 研究胶原海绵对创面愈合的影响及其对创面炎性反应干预机制.方法 将24只大鼠按笼号奇偶数随机分为对照组、实验组,每组12只,造模大鼠背部机械性全层皮肤创面,以0.9％氯化钠溶液纱布（对照组）、胶原海绵（实验组）覆盖创面,隔天更换;造模时及造模后第3、7、14、28天测定创面面积,造模后第3、7天测炎症积分,造模时及造模后第3、7天取创面组织检测TNF-d与IL-6含量.结果 治疗后7d,实验组创面面积[（0.49 ±0.08） cm2]显著小于对照组[（0.92±0.11）cm2];至28 d时,两组创面完全愈合.造模后3、7d,对照组创面肉芽炎症积分分别为（4.51±1.01）分、（2.25±0.62）分,实验组分别为（3.58±0.51）分、（1.66±0.49）分,两组差异均有统计学意义（t=2.85、2.59,均P＜0.01）.对照组造模后7 dTNF-α含量为（512 ±62）ng·L-1·g-1,高于实验组的（434±37）ng·L-1·g-1（=3.74,P ＜0.05）;对照组第3天、第7天时肉芽组织中IL-6含量分别为（2 215 ±314）ng·L-1·g-1、（1 562±174）ng·L-1·g-1,实验组分别为（1 672±161）ng·L-1·g-1、（669±75）ng · L-1·g-1,两组差异均有统计学意义（=5.33、16.33,均P＜0.01）.结论 胶原海绵可以发挥干预创面炎性反应及促进创面血管新生疗效,促进创面愈合.Objective To explore the effect of collagen sponge on wound healing and its mechanism of regulating the response of wound inflammation.Methods 24 rats were divided into the control group and the experimental group by even and odd numbers of cages,12 rats in each group.Collagen sponges were applied locally on the round mechanical full-thickness skin wounds and changed each other day as the experimental group,whereas the gauze full of saline were applied as the control group.The wound area of each wound was measured on the day of model made and the 3rd,7th,14th,28th day,thereafter,as well as the scores of inflammation response TNF-o and IL-6 levels in the wound tissues measured before and 3,7 days after model made.Results 7 days after treatment,the areas of the wound in the experimental group [（0.49 ± 0.08） cm2] was significantly smaller than that of the control group [（0.92 ± 0.11）cm2].The wounds in 2 groups were totally healed on the 28th day.On the 3rd,7th day after modeling,the score of inflammation response in the control group were about （4.51 ± 1.01） and （2.25 ± 0.62） respectively,whereas those of the experimental group were about （3.58 ± 0.51） and （1.66 ± 0.49） respectively.The differences of the two groups were statistically significant（t =2.85,2.59,all P 〈 0.01）.7 days after modeling,the amount of TNF-oin the tissues of the control group was （512 ±62）ng · L-1 · g-1,which was significantly higher than （434 ± 37） ng · L-1 · g-1 of the experimental group （t =3.74,P 〈 0.05）.The amount of IL-6 in the control group 3,7 days after modeling were （2 215 ±314）ng · L-1 · g-1 and （1 562 ± 174）ng · L-1 · g-1 respectively,which were significantly higher than （1672 ± 161）ng · L-1 · g-1 and （669 ±75）ng · L-1 · g-1 in the experimental group （t =5.33,16.33,all P 〈 0.01）.Conclusion Collagen sponge can intervene inflammatory reaction and promote the angiogenesis of the wound,promote wound healing.

关 键 词：胶原 炎症介导素类 伤口愈合 

分 类 号：R641[医药卫生—外科学]