核因子-κB在气道上皮细胞的激活及N-乙酰半胱氨酸的影响  

Activation of NF-κB in airway epithelial celland modulation mechanism of NAC

作　　者：莫红缨[1] 钟南山[1] 郑劲平[1] 龙启才[2] 

机构地区：[1]广州呼吸疾病研究所 [2]中山医科大学药理教研室,广州510120

出　　处：《中国临床药理学与治疗学》2000年第3期193-197,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：探讨在人气道上皮细胞核因子_κB(NF_κB)的激活情况及抗氧化剂N_乙酰半胱氨酸(NAC)对NF_κB的作用机理。方法应用炎前性细胞因子TNF_α刺激正常人气道上皮细胞株(16HBE)和肿瘤病人气道上皮细胞株 (H292) ,利用Western_Blot免疫印迹电泳和ELISA实验检测NF_κB的表达和IL_8的分泌水平。结果TNF_α在体外能刺激激活人气道上皮细胞16HBE和H292的NF_κB表达 ,刺激后2～4h最高 ,6h后有所下降。随着TNF_α刺激量的增加 ,NF_κB活性也随之升高。TNF_α刺激气道上皮细胞后IL_8的分泌水平增加。加入NAC后可抑制NF_κB激活 ,IL_8分泌水平随之降低 ;同时发现NAC能以一种剂量依赖方式发挥作用。结论NAC可通过阻断NF_κB激活的信号转导 ,参与细胞因子和炎症介质表达的转录调控 ,在呼吸系气道炎症控制中起着一定的作用。Aim The expression of NF-κB activation and the effect of antioxidant (N- acetylcysteine, NAC) on NF-κB activity in human airway epithelial cell was assessed. Methods Using the TNF-α,the airway epithelial cell strains of normal subject(16HBE) and tumor patient (H292) was activated and using Western-Blot and ELISA the expression of NF-κB and IL-8 were detected. Results It was found that the activity of NF-κB could be stimulated by the TNF-α and increase with the amount of TNF-α with the peak occurring at 2 to 4 hours after stimulation and then decreasing at six hours. At the same time, the level of IL-8 was elevated, but decreased with inhibition of NF-κB activity by NAC, that means the action of NAC has a dose-dependent effect. Conclusion NAC not only blocks the signal transmission activated by NF-κB, but also anticipates the transcription modulation of expression of many cell factors and inflammatory mediums. It suggests that NAC may play a role in the anti-inflammatory treatment of respiratory diseases.

关 键 词：核因子-KB 气道上皮细胞 激活 N-乙酰半胱氨酸 

分 类 号：R56[医药卫生—呼吸系统] R977.4[医药卫生—内科学]