不同来源的抗乙肝免疫核糖核酸对慢性乙型肝炎患者的免疫调节作用  

Immune modulation of anti-HBV iRNA fromdifferent sources in patients with chronic hepatitis B

作　　者：余永胜[1] 陈有华[1] 

机构地区：[1]皖南医学院弋矶山医院传染科,芜湖241001

出　　处：《中国临床药理学与治疗学》2000年第3期210-212,共3页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：安徽省重点科研项目(№99022016)

摘　　要：目的探讨HBV感染后乙肝保护抗体抗_HBs产生机体外周淋巴细胞iRNA (h_iRNA)对慢性乙型肝炎的免疫调节作用 ,并与目前单用HBsAg 免疫动物来源的抗乙肝iRNA(a_iRNA)相比较。方法利用四甲基偶氮唑盐 (MTT)法观察h_iRNA对慢性乙肝患者外周淋巴细胞HBV抗原特异增殖反应的影响。结果在HBsAg 刺激组中 ,h_iRNA、a_iRNA对慢性乙肝患者外周HB sAg 特异淋巴细胞增殖反应皆有一定程度的增强作用 ;但HBcAg 刺激组中 ,h_iRNA对慢性乙肝患者HBcAg 特异淋巴细胞增殖反应具有增强作用 ,与a_iRNA相比 ,差异有显著性意义。结论HBV感染后保护抗体产生机体外周淋巴细胞iRNA具有增强慢性乙型肝炎患者淋巴细胞对HBV抗原的特异增殖反应 ,对主要靶抗原HBcAg特异淋巴细胞增殖反应的增强作用 ,有利于清除肝内HBV。目前单用HBsAg 免疫动物的抗乙肝iRNA ,不具有对HBV其它抗原的免疫信息 ,尤其是不能对免疫细胞识别肝细胞内HBV的主要靶抗原HBcAg 产生有效免疫作用 ,可能是影响其疗效的重要原因。Aim To explore the function of immune modulation of anti-HBV iRNA in patients with chronic hepatitis B(CHB) Methods Peripheral blood lymphocyte iRNA was prepared from anti-HBs positive human body with HBV complete clearance after HBV infection(h-iRNA). The effect of h-iRNA on HBV specific lymphocyte proliferative response of peripheral lymphocyte from patients with CHB was observed by using MTT method and was compared with that by HBV specific iRNA from animal immunized only by HBsAg(a-iRNA).Results Both h-iRNA and a-iRNA increased the level of peripheral lymphocyte proliferative response to HBsAg in patients with CHB to some degree. In group of HBcAg, only h-iRNA showed its enhancement of HBcAg specific lymphocyte proliferative response.Conclusions h-iRNA can increase HBV specific lymphocyte proliferative response in patients with CHB and the function of increasing HBcAg specific lymphocyte proliterative response contributes to HBV clearance .

关 键 词：乙肝免疫核糖核酸 慢性乙型肝炎 免疫调节 淋巴细胞增殖反应 

分 类 号：R512.62[医药卫生—内科学]