青光安颗粒大鼠含药血清指纹图谱的研究  

Studies on Fingerprint of Containing Qingguang'an Granules in Rat Serum

作　　者：曾红艳[1,2] 彭清华[1] 曾志成[1,3] 李文娟[1] 彭俊[1] 李建超[1] 

机构地区：[1]湖南中医药大学中医眼科学重点学科,湖南长沙410007 [2]暨南大学医学院,广东广州510632 [3]桂阳县人民医院眼科,湖南桂阳410300

出　　处：《中华中医药学刊》2014年第7期1553-1555,I0001,I0002,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81273807);教育部博士学科点基金项目(20124323110006);湖南省自然科学基金重点项目(11JJ2050);湖南省教育厅科研基金重点项目(06A052;10A094);湖南省卫生厅科研基金项目(204084);国家中医药管理局中医眼科学重点学科建设项目;湖南省中医五官科学重点学科建设项目;湖南省研究生创新基金会重点项目(CX2013A013)

摘　　要：目的:研究青光安大鼠含药血清的指纹图谱。方法:按随机的方法将50只SD大鼠分成青光安20倍组、10倍组、5倍组、青光安2.5倍组、空白对照组。按体表面积换算的方法计算出每只大鼠的灌药量,以青光安颗粒剂溶液灌胃。灌药7天后,麻醉处死大鼠提取血清。用进样器取20μL供试品,注入高效液相仪进行指纹图谱分析。结果:色谱图的结果表明:青光安各剂量组色谱图共有特征色谱峰的相对保留时间的RSD在1%以内,相对峰面积的RSD也在3%以内,符合指纹图谱的要求。青光安20倍组与青光安2.5倍组、青光安5倍组比较两标示峰具有非常显著性差异,青光安20倍组与青光安10倍组比较两标示峰具有显著性差异;青光安10倍组与青光安2.5倍组比较两标示峰具有非常显著性差异,青光安10倍组与青光安5倍组比较两标示峰具有显著性差异;青光安5倍组与青光安2.5倍组比较两标示峰具有显著性差异。结论:青光安在体内的代谢产物浓度随给药浓度的不同也有所不同。Objective：To study the fingerprint of Qingguang＇an in rat serum.Methods：50 SD rats were radomly divided into 5 groups：Qingguang＇an 20 times group,10 times group,5 times group,2.5 times group,control group.According to the surface exchange algorithm,each group was intragastrically administrated with Qingguang＇an.7 days after administration,serum was taken out after anaesthetized.The fingerprint with chromalograms of each group was analyzed.Results：The chromatomap showed that the RSD value of relative retention time of each Qingguang＇an group＇s common peaks was within 1% and the RSD value of relative peal area was within 3% which met the standard of fingerprint.The difference of peaks between Qingguang＇an 20 times group and 2.5 times group and 5 times group were significant and the differece of peaks between Qingguang＇an 5 times group and 2.5 times group was significant.Conclusion：The concentration of Qingguang＇an metabolin varies in different drug concentration.

关 键 词：青光安颗粒剂 含药血清 指纹图谱 大鼠 

分 类 号：R285.5[医药卫生—中药学]